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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
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- Product number
- NBP2-54624 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CEACAM5/CD66e Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Clone 308/3-3 reacts with human CEACAM3 and CEACAM5, but not human CEACAM1/4/6/7/8, rat CEACAM1, or mouse CEACAM1 & CEACAM2.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Myeloma used: P3/NS1/1-Ag4.1- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C in the dark.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Detection using 10 ug/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM3/5 [308/3-3] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Immunoprecipitation utilizing 10 ug/ml Anti-CEACAM3/5 [308/3-3]. Detection of the sample using 5 ug/ml pAb Anti-CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM3/5 [308/3-3] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
