Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- UM800060 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800060, RRID:AB_2629170
- Product name
- KRT20/CK20 mouse monoclonal antibody,clone UMAB167
- Antibody type
- Monoclonal
- Description
- KRT20/CK20 mouse monoclonal antibody,clone UMAB167
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KRT20
- Isotype
- IgG
- Antibody clone number
- UMAB167
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T were transfected with 55 different plasmids of with CK cDNA (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) for 48 hrs and lysed. Cell lysates (5 ug per lane) were separated by SDS-PAGE and blotted with KRT19 ab. KRT12, 19, 25, 26, 27, 28 and 39 were positive, while all others were negative (1:2000).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of mouse tissue lysates (20ug) from Colon. Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-KRT20 mouse monoclonal antibody. (UM800060; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT20 mouse monoclonal antibody. (UM800060; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT20 mouse monoclonal antibody (UM800060). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP