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- Product number
- LS-C742078 - Provider product page
- Provider
- LSBio
- Product name
- KRT20 / CK20 / Cytokeratin 20 Antibody (clone 13F8) LS-C742078
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 13F8
- Storage
- Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Independent validation of Human KRT20 Antibody (13F8) and Human KRT20 Antibody (5G4) with Human KRT20 recombinant protein. Lane 1: 50 ng Human KRT20 recombinant protein Lane 2: 25 ng Human KRT20 recombinant protein Lane 3: 10 ng Human KRT20 recombinant protein Lane 4: 50 ng Human KRT20 recombinant protein Lane 5: 25 ng Human KRT20 recombinant protein Lane 6: 10 ng Human KRT20 recombinant protein Primary Antibody: Lane 1~3: Human KRT20 Antibody (5G4) 1 µg/ml Lane 4~6: Human KRT20 Antibody (13F8) (H&L) [IRDye800] (Licor,926-32211)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Independent validation of Human KRT20 Antibody (13F8) and Human KRT20 Antibody (5G4) with Human KRT20 recombinant protein. Lane 1: 50 ng Human KRT20 recombinant protein Lane 2: 25 ng Human KRT20 recombinant protein Lane 3: 10 ng Human KRT20 recombinant protein Lane 4: 50 ng Human KRT20 recombinant protein Lane 5: 25 ng Human KRT20 recombinant protein Lane 6: 10 ng Human KRT20 recombinant protein Primary Antibody: Lane 1~3: Human KRT20 Antibody (5G4) 1 µg/ml Lane 4~6: Human KRT20 Antibody (13F8) (H&L) [IRDye800] (Licor,926-32211)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Independent validation of Human KRT20 Antibody (13F8) and Human KRT20 Antibody (5G4) with Human KRT20 recombinant protein. Lane 1: 50 ng Human KRT20 recombinant protein Lane 2: 25 ng Human KRT20 recombinant protein Lane 3: 10 ng Human KRT20 recombinant protein Lane 4: 50 ng Human KRT20 recombinant protein Lane 5: 25 ng Human KRT20 recombinant protein Lane 6: 10 ng Human KRT20 recombinant protein Primary Antibody: Lane 1~3: Human KRT20 Antibody (5G4) 1 µg/ml Lane 4~6: Human KRT20 Antibody (13F8) (H&L) [IRDye800] (Licor,926-32211)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Standard curve of KRT20 Sandwich ELISA. The KRT20 Sandwich ELISA assay is developed by using Human KRT20 Antibody (13F8) and Biotin conjugated Human KRT20 Antibody (5G4) as capture and detect antibody, respectively. From the experimental results, Human KRT20 Antibody (13F8) and Human KRT20 Antibody (5G4) identify different epitopes. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA binding of Human KRT20 Antibody (13F8) with Human KRT20 recombinant protein. Coating antigen: KRT20, 1 µg/ml. KRT20 antibody dilutions start from 1000 ng/ml, EC50= 10.03 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Standard curve of KRT20 Sandwich ELISA. The KRT20 Sandwich ELISA assay is developed by using Human KRT20 Antibody (13F8) and Biotin conjugated Human KRT20 Antibody (5G4) as capture and detect antibody, respectively. From the experimental results, Human KRT20 Antibody (13F8) and Human KRT20 Antibody (5G4) identify different epitopes. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of Human KRT20 Antibody (13F8) with Human KRT20 recombinant protein. Coating antigen: KRT20, 1 µg/ml. KRT20 antibody dilutions start from 1000 ng/ml, EC50= 10.03 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Standard curve of KRT20 Sandwich ELISA. The KRT20 Sandwich ELISA assay is developed by using Human KRT20 Antibody (13F8) and Biotin conjugated Human KRT20 Antibody (5G4) as capture and detect antibody, respectively. From the experimental results, Human KRT20 Antibody (13F8) and Human KRT20 Antibody (5G4) identify different epitopes. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of Human KRT20 Antibody (13F8) with Human KRT20 recombinant protein. Coating antigen: KRT20, 1 µg/ml. KRT20 antibody dilutions start from 1000 ng/ml, EC50= 10.03 ng/ml.
