Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- MA1-06401 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Desmin Monoclonal Antibody (D9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-06401 detects desmin in human, mouse, rat, rabbits, pig, and chicken samples.
- Antibody clone number
- D9
- Concentration
- 1 mg/mL
Submitted references Multiple benign fibrous histiocytomas of the mandible: A case report and review of the literature.
The RNA-binding protein HuR is a negative regulator in adipogenesis.
Spontaneous Urinary Bladder Leiomyoma in a Rhesus Macaque (Macaca mulatta).
Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle.
Wang Y, Huang Y, Cai WX, Tao Q
Experimental and therapeutic medicine 2022 Sep;24(3):593
Experimental and therapeutic medicine 2022 Sep;24(3):593
The RNA-binding protein HuR is a negative regulator in adipogenesis.
Siang DTC, Lim YC, Kyaw AMM, Win KN, Chia SY, Degirmenci U, Hu X, Tan BC, Walet ACE, Sun L, Xu D
Nature communications 2020 Jan 10;11(1):213
Nature communications 2020 Jan 10;11(1):213
Spontaneous Urinary Bladder Leiomyoma in a Rhesus Macaque (Macaca mulatta).
Scott KE, Frydman G, Fox JG, Bakthavatchalu V
Comparative medicine 2018 Jun 1;68(3):243-247
Comparative medicine 2018 Jun 1;68(3):243-247
Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle.
Lesmana R, Sinha RA, Singh BK, Zhou J, Ohba K, Wu Y, Yau WW, Bay BH, Yen PM
Endocrinology 2016 Jan;157(1):23-38
Endocrinology 2016 Jan;157(1):23-38
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Desmin Monoclonal Antibody (D9) (Product # MA1-06401) and a 55 kDa bands corresponding to Desmin was observed in Rat Heart but not in Rat Liver, Rat Kidney, Rat Brain and Rat Spleen which are reported to be negative or low expressing tissues. An uncharacterized band of ~25 kDa was also observed in tissues. Tissue extracts (30 µg lysate) of Rat Heart (Lane 1), Rat Liver (Lane 2), Rat Kidney (Lane 3), Rat Brain (Lane 4) and Rat Spleen (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on frozen sections of swine colon showing staining of muscle tissues stained with Desmin monoclonal antibody (Product # MA1-06401).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on frozen sections of swine stomach showing staining of muscle tissues stained with Desmin monoclonal antibody (Product # MA1-06401).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pathological examination of the tumors. (A) Histology indicated that the tumors consisted of spindle-shaped cells and collagen fibers, and the former were arranged in a storiform pattern and surrounded by the latter. The arrows a and b indicated the collagen fiber and cell, respectively. (H&E staining; magnification, x200). Immunohistochemistry suggested that the tumor cells were strongly positive for (B) CD68 and (C) XIIIa factor, while they were weakly positive for (D) alpha-SMA and (E) desmin. (F) The tumor cells were negative for CD34 (magnification, x200; scale bars, 100 um).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Deletion of HuR in BAT promotes brown adipogenesis. a GSEA on HuR-FKO and Ctrl BAT indicated that myogenesis pathway was significantly downregulated in HuR knockout BAT. b Real-time PCR to confirm the expression of BAT-selective markers, adipogenesis, lipogenesis (Ctrl, n = 6; HuR-FKO, n = 7), and c muscle markers (Ctrl, n = 6; HuR-FKO, n = 8). d Western blot to exam the protein level of Ucp1, Pgc1a, and Desmin in BAT from HuR-FKO and Ctrl mice. e Western blot analysis of HuR expression in BAT, iWAT, and eWAT of HuR-BATKO and control mice. f Morphological characteristics of HuR-BATKO and control littermates by H&E staining. Scale bars represent 100 muM. g Real-time PCR to confirm the expression of genes for BAT-selective markers and adipogenesis in BAT from HuR-BATKO and Ctrl mice (Ctrl, n = 6; HuR-BATKO, n = 7). Error bars are mean +- SEM. Statistical significance was determined by Student's t -test; * p < 0.05. Source data are provided as a Source Data file.