PA1-37555

antibody from Invitrogen Antibodies

Targeting: DES CMD1I, CSM1, CSM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-37555

Provider

Invitrogen Antibodies

Product name

Anti-Desmin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and rat based on sequence homology. A suggested positive control is skeletal muscle or sarcoma.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

7 mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Desmin Polyclonal Antibody (Product # PA1-37555) and a 55 kDa bands corresponding to Desmin was observed in Mouse Skeletal Muscle, Rat Skeletal Muscle, Mouse Heart, Rat Heart, Cardiomyocytes and RD cell line but not in Mouse Kidney, iPSC and MCF7 cell line. An isoform at 45 kDa was also observed in Cardiomyocyte and RD. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Kidney (Lane 7), whole cell extracts (30 µg lysate) of iPSC (Lane 5), Cardiomyocytes (Lane 6), RD (Lane 8) and MCF7 (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).