- PA1-37556 - Invitrogen Antibodies DES antibody

Submitted references Postnatal, ontogenic liver growth accomplished by biliary/oval cell proliferation and differentiation.

Szücs A, Paku S, Sebestyén E, Nagy P, Dezső K
PloS one 2020;15(5):e0233736

Caveolae in CNS arterioles mediate neurovascular coupling.

Chow BW, Nuñez V, Kaplan L, Granger AJ, Bistrong K, Zucker HL, Kumar P, Sabatini BL, Gu C
Nature 2020 Mar;579(7797):106-110

Resolving Discrepant Findings on ANGPTL8 in β-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversy.

Cox AR, Barrandon O, Cai EP, Rios JS, Chavez J, Bonnyman CW, Lam CJ, Yi P, Melton DA, Kushner JA
PloS one 2016;11(7):e0159276

Rosmarinic acid and baicalin epigenetically derepress peroxisomal proliferator-activated receptor γ in hepatic stellate cells for their antifibrotic effect.

Yang MD, Chiang YM, Higashiyama R, Asahina K, Mann DA, Mann J, Wang CC, Tsukamoto H
Hepatology (Baltimore, Md.) 2012 Apr;55(4):1271-81

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-Desmin Polyclonal Antibody (Product # PA1-37556) and a 55 kDa band corresponding to Desmin was observed in Mouse Skeletal Muscle, Rat Skeletal Muscle, Mouse Heart, Rat Heart, Cardiomyocytes and RD cell line but not in Mouse Kidney, iPSC and MCF7 cell line. Two isoforms at ~40, ~50 kDa were also observed in Mouse Skeletal Muscle, Cardiomyocytes and RD cell line. An uncharacterized band of ~70 kDa was also observed in Cardiomyocytes. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Kidney (Lane 7) and Whole cell extracts (30 µg lysate) of iPSC (Lane 5), Cardiomyocytes (Lane 6), RD (Lane 8) and MCF7 (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of Desmin (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5 x 105 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Desmin polyclonal antibody (Product # PA1-37556) at dilution of 1:300 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were visualized using Hoechst 33342 (Product # 62249). Images were taken at 20X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis Desmin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Desmin Polyclonal Antibody (Product PA1-37556) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of Desmin using a polyclonal antibody (Product # PA1-37556).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 7 Highly proliferative cells in islets of ANGPTL8 treated mice are not glucagon, endocrine, epithelial, neuronal, myofibroblast, or vascular cells. (a) Immunostaining for insulin (yellow), glucagon (green), EdU (red) and DAPI (blue). (b) Immunostaining for synaptophysin (green), EdU (red), and DAPI (blue). (c-h) Immunostaining for EdU (green) and DAPI (blue) with various makers (red); (c) E-cadherin, (d) N-cadherin, (e) smooth muscle actin alpha (SMAalpha), (f) desmin, (g) CD31, (h) CD34. Insets indicate EdU positive cells that do not express glucagon, synaptophysin, E-cadherin, N-cadherin, SMAalpha, desmin, CD31, or CD34. Scale bar = 100 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 3 Characterization of bile ducts (oval cells) and small hepatocytes on 10 th days of the AAF/CA treatment. A. Bouin's fixed section stained for AFP. AFP positive B/OC ductules spread from the portal area into the parenchyma. Scale bar: 50 mum. B. Frozen section stained for OV6 (red) and DLK1 (green). Numerous DLK1 positive cells are situated within the OV6 positive B/OC ductules. Scale bar: 50 mum. C. Frozen section stained for laminin (red) and Desmin (green). Laminin positive basement membrane framed ductules accompanied by desmin positive cells spread from the portal area into the parenchyma. Inset shows typical ""U"" shaped (arrowhead) termination of B/OC ductules on hepatocytes. Desmin positive myofibroblasts are closely associated with the laminin positive basement membrane. PV; Portal vein. Scale bar: 200 mum, Scale bar for inset: 25 mum. D. Frozen section stained for laminin (red) and SMA (green). Laminin positive basement membrane framed ductules accompanied by SMA positive cells spread from the portal area into the parenchyma. Inset as before shows typical ""U"" shaped (arrowheads) termination of B/OC ductules on hepatocytes. SMA, another established marker of myofibroblasts is also closely associated with the laminin positive basement membrane. PV; Portal vein. Scale bar: 200 mum, Scale bar for inset: 25 mum. E. Frozen section stained with streptavidin-TRITC to detect endogenous biotin. Group of small hepatocytes characterized by low endogenous biotin content a

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

PA1-37556

Antibody data