A-21282

antibody from Invitrogen Antibodies

Targeting: GFAP FLJ45472

Antibody data

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Validation data

GFAP protein structure - A-21282 shown in red.

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 Centrally administered XPro1595 improves motor functions and decreases lesion size after SCI. (A) Analysis of BMS scores in mice treated centrally for three consecutive days with either saline, XPro1595, or etanercept showed that XPro1595-treated mice significantly improved their BMS score from 3 to 35 days after SCI compared to both saline- and etanercept-treated mice (* P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 Expression of total MeCP2 and MeCP2E1 in primary neurons and astrocytes. A ) Expression of total MeCP2 in embryonic primary cortical neurons and astrocytes are detected by immunofluorescence labelling. Cells were labelled with beta-III tubulin (beta TUB III) and GFAP to mark neurons and astrocytes, respectively. B ) Expression of MeCP2E1 in primary cortical neurons and astrocytes. Cells were labelled with NEUN and GFAP to mark neurons and astrocytes, respectively. Scale bars represent 5 um. C ) Western blot analysis of MeCP2E1 levels in primary cortical neurons and astrocytes. The graph depicts the quantification of MeCP2E1 in neurons and astrocytes, p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4. Collages of 1D Periodic Cytoskeletal Motifs on the 2D Membranes of Developing NSCs. (A) 3D-STORM image of betaII spectrin (C terminus) at the dorsal (top) membrane of an NSC in transition to a neuron. (B) Overlapped epifluorescence of the neuron marker Tuj (neuron-specific class III beta-tubulin; red) and betaII spectrin (green) of the cell. (C) One-dimensional autocorrelations along the green, magenta, and cyan boxes in (A). Gray grid lines mark multiples of 180 nm. (D) 3D-STORM image of betaII spectrin (C terminus) at the ventral (bottom) membrane of an NSC in transition to an astrocyte. (E) GFAP (red) and betaII spectrin (green) staining of the cell. (F) One-dimensional autocorrelations along the green, magenta, and orange boxes in (D). Gray grid lines mark multiples of 180 nm. See also Figure S4 .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. The Membrane Cytoskeletons of NSC-Derived Astrocytes and Oligodendrocytes Are Highly Periodic. (A) 3D-STORM image of the phalloidin-labeled actin cytoskeleton of an NSC-derived astrocyte. Inset: enlarged image of a process. (B) Overlapped epifluorescence of actin (green) and GFAP (red). (C) One-dimensional autocorrelation along the red box in (A). Gray grid lines mark multiples of 180 nm. (D) 3D-STORM image of immunolabeled adducin for an NSC-derived oligodendrocyte. Inset: enlarged image of a process. (E) MBP staining of the cell. (F) One-dimensional autocorrelations along the orange, magenta, and cyan boxes in (D). Gray grid lines mark multiples of 180 nm. See also Figures S2 and S3 .