R31508

antibody from NSJ Bioreagents

Targeting: GFAP FLJ45472

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31508

Provider

NSJ Bioreagents

Product name

GFAP Antibody

Antibody type

Polyclonal

Antigen

Human partial recombinant protein (AA 93-432) was used as the immunogen for this GFAP antibody.

Description

Antigen affinity purified antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

Lyophilized; resuspend with 200 ul for 0.5 mg/ml

Storage

After reconstitution, the GFAP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of GFAP antibody and Lane 1: rat brain; 2: mouse brain; 3: U87; 4: SHG-44 (human glioma line); 5: NEURO; 6: HeLa lysate

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GFAP antibody testing of human brain cancer

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GFAP antibody testing of mouse brain tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GFAP antibody testing of rat brain tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE mouse brain with GFAP antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.