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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA1-10046 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Actin Muscle Monoclonal Antibody (HHF35)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.
- Antibody clone number
- HHF35
- Concentration
- 1 mg/mL
Submitted references Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess.
Expression of E-cadherin in angiomyolipoma.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?
G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.
Chorangiosis: the potential role of smoking and air pollution.
Myointimoma of the glans penis.
Microfabrication-based modulation of embryonic stem cell differentiation.
Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.
Ectopic cervical anaplastic ependymoma.
Epstein-Barr virus-associated smooth muscle tumour: a distinctive mesenchymal tumour of immunocompromised individuals.
Lee PW, Yau DT, Lau PP, Chan JK
International journal of surgical pathology 2014 May;22(3):286-90
International journal of surgical pathology 2014 May;22(3):286-90
Expression of E-cadherin in angiomyolipoma.
Wang Z, Gong Q, Fan Q
Human pathology 2012 Dec;43(12):2348-53
Human pathology 2012 Dec;43(12):2348-53
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?
Eloy C, Vinagre J, Cameselle-Teijeiro J, Paiva ME, Soares P, Sobrinho-Simões M
International journal of surgical pathology 2011 Apr;19(2):276-80
International journal of surgical pathology 2011 Apr;19(2):276-80
G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.
Shimoji K, Yuasa S, Onizuka T, Hattori F, Tanaka T, Hara M, Ohno Y, Chen H, Egasgira T, Seki T, Yae K, Koshimizu U, Ogawa S, Fukuda K
Cell stem cell 2010 Mar 5;6(3):227-37
Cell stem cell 2010 Mar 5;6(3):227-37
Chorangiosis: the potential role of smoking and air pollution.
Akbulut M, Sorkun HC, Bir F, Eralp A, Duzcan E
Pathology, research and practice 2009;205(2):75-81
Pathology, research and practice 2009;205(2):75-81
Myointimoma of the glans penis.
Vardar E, Gunlusoy B, Arslan M, Kececi S
Pathology international 2007 Mar;57(3):158-61
Pathology international 2007 Mar;57(3):158-61
Microfabrication-based modulation of embryonic stem cell differentiation.
Park J, Cho CH, Parashurama N, Li Y, Berthiaume F, Toner M, Tilles AW, Yarmush ML
Lab on a chip 2007 Aug;7(8):1018-28
Lab on a chip 2007 Aug;7(8):1018-28
Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.
Boudova L, Kazakov DV, Sima R, Vanecek T, Torlakovic E, Lamovec J, Kutzner H, Szepe P, Plank L, Bouda J, Hes O, Mukensnabl P, Michal M
The American Journal of dermatopathology 2005 Oct;27(5):375-86
The American Journal of dermatopathology 2005 Oct;27(5):375-86
Ectopic cervical anaplastic ependymoma.
Wang Z, Huang G, Yan P, Liang R, Wang J, Yan Q, Zhang J, Cheng H, Hu P, Ma MJ
Pathology international 2005 Dec;55(12):781-4
Pathology international 2005 Dec;55(12):781-4
Epstein-Barr virus-associated smooth muscle tumour: a distinctive mesenchymal tumour of immunocompromised individuals.
Cheuk W, Li PC, Chan JK
Pathology 2002 Jun;34(3):245-9
Pathology 2002 Jun;34(3):245-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Rat Colon (Lane 6), Rat Pancreas (Lane 7), Mouse Kidney (Lane 8) and whole cell extract (30 µg lysate) of C2C12 (Lane 5). The blot was probed with Anti-Actin Muscle Monoclonal Antibody (HHF35) (Product # MA1-10046, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to beta Actin was observed in muscle specific tissues and cell line when compared to non muscle tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35 (Product # MA1-10046). Lane 1: murine femoral muscle. Lane 2: murine heart. Lane 3: HeLa. Lane 4: HUVEC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human smooth muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35) Monoclonal antibody (Product # MA1-10046).
