NBP2-52922

antibody from Novus Biologicals

Targeting: P4HA2 C-P4Halpha(II)

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-52922

Provider

Novus Biologicals

Product name

Mouse Monoclonal P4HA2 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human P4HA2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

NTDWPALEDLVLQDS


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human epididymis shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.