NBP2-47635-0.1mg

antibody from Novus Biologicals

Targeting: ENO2

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP2-47635-0.1mg - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal Enolase 2/Neuron-specific Enolase Antibody
Antibody type: Monoclonal
Description: Protein A or G purified. Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Concentration: 0.2 mg/ml
Storage: Store at 4C.

ENO2 protein structure - NBP2-47635-0.1mg shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - An intracellular stain was performed on A549 cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Azide and BSA Free format of this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - An intracellular stain was performed on SH-SY5Y cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Azide and BSA Free format of this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - An intracellular stain was performed on HeLa cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image from the Allophycocyanin version of this antibody.