NBP2-54452AF700

antibody from Novus Biologicals

Targeting: ENO2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-54452AF700

Provider

Novus Biologicals

Product name

Mouse Monoclonal Enolase 2/Neuron-specific Enolase Antibody

Antibody type

Monoclonal

Description

Protein A or G purified. Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Near infrared dye

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C in the dark.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [Alexa Fluor® 700] [NBP2-54452AF700] - An intracellular stain was performed on A549 cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.