Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA803185 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA803185, RRID:AB_2626702
- Product name
- NKX2-1 mouse monoclonal antibody, clone OTI3F3 (formerly 3F3)
- Antibody type
- Monoclonal
- Description
- NKX2-1 mouse monoclonal antibody, clone OTI3F3 (formerly 3F3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NKX2-1
- Isotype
- IgG
- Antibody clone number
- OTI3F3
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKX2-1 (RC217520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKX2-1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-NKX2-1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of NCI-H1975 cells using anti-NKX2-1 mouse monoclonal antibody (TA803185). The right is A549 cells as negative control.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of TT cells using anti-NKX2-1 mouse monoclonal antibody (TA803185). The right is A549 cells as negative control.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-NKX2-1 mouse monoclonal antibody. (TA803185)
- Validation comment
- IHC