Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX109636 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109636, RRID:AB_1950966
- Product name
- c-Myc antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.
GRP78/BiP is a novel downstream target of IGF-1 receptor mediated signaling.
Areca nut induces miR-23a and inhibits repair of DNA double-strand breaks by targeting FANCG.
Tsai CH, Lin LT, Wang CY, Chiu YW, Chou YT, Chiu SJ, Wang HE, Liu RS, Wu CY, Chan PC, Yang MH, Chiou SH, Liao MJ, Lee YJ
Biochimica et biophysica acta 2015 May;1852(5):851-61
Biochimica et biophysica acta 2015 May;1852(5):851-61
GRP78/BiP is a novel downstream target of IGF-1 receptor mediated signaling.
Pfaffenbach KT, Pong M, Morgan TE, Wang H, Ott K, Zhou B, Longo VD, Lee AS
Journal of cellular physiology 2012 Dec;227(12):3803-11
Journal of cellular physiology 2012 Dec;227(12):3803-11
Areca nut induces miR-23a and inhibits repair of DNA double-strand breaks by targeting FANCG.
Tsai YS, Lin CS, Chiang SL, Lee CH, Lee KW, Ko YC
Toxicological sciences : an official journal of the Society of Toxicology 2011 Oct;123(2):480-90
Toxicological sciences : an official journal of the Society of Toxicology 2011 Oct;123(2):480-90
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE GTX109636 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX109636 diluted at 1:3000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- c-Myc antibody [N1], N-term detects c-Myc protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: c-Myc protein stained by c-Myc antibody [N1], N-term (GTX109636) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry on human embryonic stem cells, staining with c-Myc (GTX109636)antibody at 1:100 dilution(purple) or rabbit IgG (black).
- Validation comment
- FACS
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MYC antibody immunoprecipitates MYC protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 ?g preimmune rabbit IgGB. 5 ?g of MYC antibody (GTX109636)The precipitated DNA was detected by PCR with primer set targeting to mSHMT promoter.
- Validation comment
- ChIP