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antibody from Invitrogen Antibodies
Targeting: MYC
bHLHe39, c-Myc, MYCC
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- Antibody Data
- Antigen structure
- References [152]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [3]
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- Product number
- 13-2500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Myc Monoclonal Antibody (9E10)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9E10
- Vial size
- 200 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references USP25 inhibition ameliorates Alzheimer's pathology through the regulation of APP processing and Aβ generation.
Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome.
Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis.
Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis.
A network of Gα(i) signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEF.
TRPV2-spike protein interaction mediates the entry of SARS-CoV-2 into macrophages in febrile conditions.
Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion.
Paramyxovirus-Like Particles as Protein Delivery Vehicles.
Highly Conserved C-Terminal Region of Indian Hedgehog N-Fragment Contributes to Its Auto-Processing and Multimer Formation.
The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.
Interactome Mapping of eIF3A in a Colon Cancer and an Immortalized Embryonic Cell Line Using Proximity-Dependent Biotin Identification.
TMEM41B and VMP1 are scramblases and regulate the distribution of cholesterol and phosphatidylserine.
P2X7 Receptors and TMEM16 Channels Are Functionally Coupled with Implications for Macropore Formation and Current Facilitation.
Persistently Elevated mTOR Complex 1-S6 Kinase 1 Disrupts DARPP-32-Dependent D(1) Dopamine Receptor Signaling and Behaviors.
Kog1/Raptor mediates metabolic rewiring during nutrient limitation by controlling SNF1/AMPK activity.
Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration.
TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction.
Correction: Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
TRIM47 Promotes the Development of Glioma by Ubiquitination and Degradation of FOXO1.
A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodies.
Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.
The single CCA-adding enzyme of T. brucei has distinct functions in the cytosol and in mitochondria.
Directed RNase H Cleavage of Nascent Transcripts Causes Transcription Termination.
The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7.
ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation.
Genomic deregulation of PRMT5 supports growth and stress tolerance in chronic lymphocytic leukemia.
Argonaute binding within 3'-untranslated regions poorly predicts gene repression.
The yeast cell wall protein Pry3 inhibits mating through highly conserved residues within the CAP domain.
Yeast surface display of full-length human microtubule-associated protein tau.
Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis.
Image-Based Analysis of Protein Stability.
A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans.
Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027.
Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.
Molecular architecture of the Bardet-Biedl syndrome protein 2-7-9 subcomplex.
Engineering of anti-human interleukin-4 receptor alpha antibodies with potent antagonistic activity.
The LIM protein Ajuba recruits DBC1 and CBP/p300 to acetylate ERα and enhances ERα target gene expression in breast cancer cells.
Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli.
Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B.
Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.
The Circadian tau Mutation in Casein Kinase 1 Is Part of a Larger Domain That Can Be Mutated to Shorten Circadian Period.
Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication.
Involvement of PDZ-SAP97 interactions in regulating AQP2 translocation in response to vasopressin in LLC-PK(1) cells.
The binding of Chp2's chromodomain to methylated H3K9 is essential for Chp2's role in heterochromatin assembly in fission yeast.
Structural basis for arginine glycosylation of host substrates by bacterial effector proteins.
A biosensor for MAPK-dependent Lin28 signaling.
C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis.
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function.
Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes.
Molecular basis of titin exon exclusion by RBM20 and the novel titin splice regulator PTB4.
Bmp2 and Notch cooperate to pattern the embryonic endocardium.
A biosensor-based framework to measure latent proteostasis capacity.
Interaction between ROR1 and MuSK activation complex in myogenic cells.
Inflachromene inhibits autophagy through modulation of Beclin 1 activity.
SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity.
High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles.
Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling.
Charting organellar importomes by quantitative mass spectrometry.
Clinical-scale expansion of CD34(+) cord blood cells amplifies committed progenitors and rapid scid repopulation cells.
Dentin sialoprotein facilitates dental mesenchymal cell differentiation and dentin formation.
An engineered protein antagonist of K-Ras/B-Raf interaction.
Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei.
Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1.
Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease.
Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei.
A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase.
PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.
Drosophila Mitf regulates the V-ATPase and the lysosomal-autophagic pathway.
Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism.
Impaired ADAMTS9 secretion: A potential mechanism for eye defects in Peters Plus Syndrome.
The NPC2 protein: A novel dog allergen.
cMyc-p53 feedback mechanism regulates the dynamics of T lymphocytes in the immune response.
Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.
Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion.
The LIM protein Ajuba promotes adipogenesis by enhancing PPARγ and p300/CBP interaction.
The levels of epithelial anchor proteins β-catenin and zona occludens-1 are altered by E7 of human papillomaviruses 5 and 8.
The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins.
Outer membrane protein functions as integrator of protein import and DNA inheritance in mitochondria.
The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein.
Delineating the Tes Interaction Site in Zyxin and Studying Cellular Effects of Its Disruption.
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
Ajuba Preferentially Binds LXRα/RXRγ Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.
Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy.
Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances.
The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units.
Maternal dazap2 Regulates Germ Granules by Counteracting Dynein in Zebrafish Primordial Germ Cells.
Structures of the Gβ-CCT and PhLP1-Gβ-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγ dimer assembly.
Novel components of the Toxoplasma inner membrane complex revealed by BioID.
Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG.
The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling.
RON5 is critical for organization and function of the Toxoplasma moving junction complex.
Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells.
Role of phosphatidylinositol 4,5-bisphosphate in regulating EHD2 plasma membrane localization.
The human minor histocompatibility antigen 1 is a RhoGAP.
CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire.
Determinants at the N- and C-termini of Gα12 required for activation of Rho-mediated signaling.
Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells.
Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation.
The ATM substrate KAP1 controls DNA repair in heterochromatin: regulation by HP1 proteins and serine 473/824 phosphorylation.
Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts.
The F-BAR protein PACSIN2 regulates epidermal growth factor receptor internalization.
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Interactome mapping suggests new mechanistic details underlying Alzheimer's disease.
Array-based comparative genomic hybridization identifies CDK4 and FOXM1 alterations as independent predictors of survival in malignant peripheral nerve sheath tumor.
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
Aurora-A phosphorylates, activates, and relocalizes the small GTPase RalA.
Ribosomal protein L11 associates with c-Myc at 5 S rRNA and tRNA genes and regulates their expression.
Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis.
Classic and desmoplastic medulloblastoma: complete case reports and characterizations of two new cell lines.
Regulatory mechanism of matrix metalloprotease-2 enzymatic activity by factor Xa and thrombin.
An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization.
Immunohistochemical analysis of the progression of flat and papillary preneoplastic lesions in intrahepatic cholangiocarcinogenesis in hepatolithiasis.
Regulation of ADAMTS9 secretion and enzymatic activity by its propeptide.
Analysis of a Shc family adaptor protein, ShcD/Shc4, that associates with muscle-specific kinase.
The prolyl isomerase Pin1 affects Che-1 stability in response to apoptotic DNA damage.
Kinetic mechanism of the fastest motor protein, Chara myosin.
Mutations in PDLIM3 and MYOZ1 encoding myocyte Z line proteins are infrequently found in idiopathic dilated cardiomyopathy.
The binding of DYNLL2 to myosin Va requires alternatively spliced exon B and stabilizes a portion of the myosin's coiled-coil domain.
Activation of transferrin receptor 1 by c-Myc enhances cellular proliferation and tumorigenesis.
AP-1 differentially expressed proteins Krp1 and fibronectin cooperatively enhance Rho-ROCK-independent mesenchymal invasion by altering the function, localization, and activity of nondifferentially expressed proteins.
Characterization of the human GARP (Golgi associated retrograde protein) complex.
Function and regulation of Tumbleweed (RacGAP50C) in neuroblast proliferation and neuronal morphogenesis.
Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE.
Functional properties of the p33 and p55 domains of the Helicobacter pylori vacuolating cytotoxin.
Akt phosphorylates Tal1 oncoprotein and inhibits its repressor activity.
Proteolytic maturation and activation of autotaxin (NPP2), a secreted metastasis-enhancing lysophospholipase D.
Trapping prion protein in the endoplasmic reticulum impairs PrPC maturation and prevents PrPSc accumulation.
Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1.
Distinct roles of EGF repeats for the Notch signaling system.
Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin.
Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration.
HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.
WW domain-containing proteins, WWOX and YAP, compete for interaction with ErbB-4 and modulate its transcriptional function.
A role for Ubc9 in tumorigenesis.
Isoforms of the polarity protein par6 have distinct functions.
Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle.
A homologue of Cdk8 is required for spore cell differentiation in Dictyostelium.
Expression of c-erbB2, p53, Bcl-2, Bax, c-myc and Ki-67 in apocrine metaplasia and apocrine change within sclerosing adenosis of the breast.
Transendothelial migration of colon carcinoma cells requires expression of E-selectin by endothelial cells and activation of stress-activated protein kinase-2 (SAPK2/p38) in the tumor cells.
Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic.
Involvement of phospholipase D in sphingosine 1-phosphate-induced activation of phosphatidylinositol 3-kinase and Akt in Chinese hamster ovary cells overexpressing EDG3.
Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin.
PYK2 as a mediator of endothelin-1/G alpha 11 signaling to GLUT4 glucose transporters.
Leucine zipper-mediated homodimerization of the p21-activated kinase-interacting factor, beta Pix. Implication for a role in cytoskeletal reorganization.
p59fyn tyrosine kinase associates with multiple T-cell receptor subunits through its unique amino-terminal domain.
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Zheng Q, Song B, Li G, Cai F, Wu M, Zhao Y, Jiang L, Guo T, Shen M, Hou H, Zhou Y, Zhao Y, Di A, Zhang L, Zeng F, Zhang XF, Luo H, Zhang X, Zhang H, Zeng Z, Huang TY, Dong C, Qing H, Zhang Y, Zhang Q, Wang X, Wu Y, Xu H, Song W, Wang X
The Journal of clinical investigation 2022 Mar 1;132(5)
The Journal of clinical investigation 2022 Mar 1;132(5)
Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome.
Li S, Li W, Yuan J, Bullova P, Wu J, Zhang X, Liu Y, Plescher M, Rodriguez J, Bedoya-Reina OC, Jannig PR, Valente-Silva P, Yu M, Henriksson MA, Zubarev RA, Smed-Sörensen A, Suzuki CK, Ruas JL, Holmberg J, Larsson C, Christofer Juhlin C, von Kriegsheim A, Cao Y, Schlisio S
Nature metabolism 2022 Jun;4(6):739-758
Nature metabolism 2022 Jun;4(6):739-758
Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis.
Li L, Freitag J, Asbrand C, Munteanu B, Wang BT, Zezina E, Didier M, Thill G, Rocher C, Herrmann M, Biesemann N
Scientific reports 2022 Jul 12;12(1):11830
Scientific reports 2022 Jul 12;12(1):11830
Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis.
Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X
Cell discovery 2022 Jan 18;8(1):3
Cell discovery 2022 Jan 18;8(1):3
A network of Gα(i) signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEF.
Chandan NR, Abraham S, SenGupta S, Parent CA, Smrcka AV
Science signaling 2022 Jan 18;15(717):eabi9869
Science signaling 2022 Jan 18;15(717):eabi9869
TRPV2-spike protein interaction mediates the entry of SARS-CoV-2 into macrophages in febrile conditions.
Xu J, Yang Y, Hou Z, Jia H, Wang Y
Theranostics 2021;11(15):7379-7390
Theranostics 2021;11(15):7379-7390
Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion.
Kim YH, Kwak MS, Lee B, Shin JM, Aum S, Park IH, Lee MG, Shin JS
Autophagy 2021 Sep;17(9):2345-2362
Autophagy 2021 Sep;17(9):2345-2362
Paramyxovirus-Like Particles as Protein Delivery Vehicles.
Panthi S, Schmitt PT, Lorenz FJ, Stanfield BA, Schmitt AP
Journal of virology 2021 Sep 27;95(20):e0103021
Journal of virology 2021 Sep 27;95(20):e0103021
Highly Conserved C-Terminal Region of Indian Hedgehog N-Fragment Contributes to Its Auto-Processing and Multimer Formation.
Wang X, Liu H, Liu Y, Han G, Wang Y, Chen H, He L, Ma G
Biomolecules 2021 May 25;11(6)
Biomolecules 2021 May 25;11(6)
The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.
Yamada Y, Schaap P
Autophagy 2021 Mar;17(3):640-655
Autophagy 2021 Mar;17(3):640-655
Interactome Mapping of eIF3A in a Colon Cancer and an Immortalized Embryonic Cell Line Using Proximity-Dependent Biotin Identification.
Vo DK, Engler A, Stoimenovski D, Hartig R, Kaehne T, Kalinski T, Naumann M, Haybaeck J, Nass N
Cancers 2021 Mar 14;13(6)
Cancers 2021 Mar 14;13(6)
TMEM41B and VMP1 are scramblases and regulate the distribution of cholesterol and phosphatidylserine.
Li YE, Wang Y, Du X, Zhang T, Mak HY, Hancock SE, McEwen H, Pandzic E, Whan RM, Aw YC, Lukmantara IE, Yuan Y, Dong X, Don A, Turner N, Qi S, Yang H
The Journal of cell biology 2021 Jun 7;220(6)
The Journal of cell biology 2021 Jun 7;220(6)
P2X7 Receptors and TMEM16 Channels Are Functionally Coupled with Implications for Macropore Formation and Current Facilitation.
Dunning K, Martz A, Peralta FA, Cevoli F, Boué-Grabot E, Compan V, Gautherat F, Wolf P, Chataigneau T, Grutter T
International journal of molecular sciences 2021 Jun 18;22(12)
International journal of molecular sciences 2021 Jun 18;22(12)
Persistently Elevated mTOR Complex 1-S6 Kinase 1 Disrupts DARPP-32-Dependent D(1) Dopamine Receptor Signaling and Behaviors.
Lin R, Learman LN, Na CH, Renuse S, Chen KT, Chen PY, Lee GH, Xiao B, Resnick SM, Troncoso JC, Szumlinski KK, Linden DJ, Park JM, Savonenko A, Pandey A, Worley PF
Biological psychiatry 2021 Jun 1;89(11):1058-1072
Biological psychiatry 2021 Jun 1;89(11):1058-1072
Kog1/Raptor mediates metabolic rewiring during nutrient limitation by controlling SNF1/AMPK activity.
Rashida Z, Srinivasan R, Cyanam M, Laxman S
Science advances 2021 Apr;7(16)
Science advances 2021 Apr;7(16)
Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration.
Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
Current biology : CB 2021 Apr 12;31(7):1521-1530.e8
Current biology : CB 2021 Apr 12;31(7):1521-1530.e8
TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction.
Li B, Niswander LA
Frontiers in cell and developmental biology 2020;8:599890
Frontiers in cell and developmental biology 2020;8:599890
Correction: Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
PLOS ONE Staff
PloS one 2020;15(5):e0234022
PloS one 2020;15(5):e0234022
Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
Azarakhsh M, Rumyantsev AM, Lebedeva MA, Lutova LA
PloS one 2020;15(4):e0232352
PloS one 2020;15(4):e0232352
TRIM47 Promotes the Development of Glioma by Ubiquitination and Degradation of FOXO1.
Wei H, Ding C, Zhuang H, Hu W
OncoTargets and therapy 2020;13:13401-13411
OncoTargets and therapy 2020;13:13401-13411
A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodies.
Chen X, Gentili M, Hacohen N, Regev A
bioRxiv : the preprint server for biology 2020 Oct 30;
bioRxiv : the preprint server for biology 2020 Oct 30;
Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Gutierrez A, Kim JO, Umbreit NT, Asbury CL, Davis TN, Miller MP, Biggins S
Current biology : CB 2020 Nov 16;30(22):4491-4499.e5
Current biology : CB 2020 Nov 16;30(22):4491-4499.e5
CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.
Gurkaslar HK, Culfa E, Arslanhan MD, Lince-Faria M, Firat-Karalar EN
Cell reports 2020 May 12;31(6):107630
Cell reports 2020 May 12;31(6):107630
The single CCA-adding enzyme of T. brucei has distinct functions in the cytosol and in mitochondria.
Shikha S, Schneider A
The Journal of biological chemistry 2020 May 1;295(18):6138-6150
The Journal of biological chemistry 2020 May 1;295(18):6138-6150
Directed RNase H Cleavage of Nascent Transcripts Causes Transcription Termination.
Lai F, Damle SS, Ling KK, Rigo F
Molecular cell 2020 Mar 5;77(5):1032-1043.e4
Molecular cell 2020 Mar 5;77(5):1032-1043.e4
The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7.
Morel M, Shah KN, Long W
The Journal of biological chemistry 2020 Jun 5;295(23):7970-7980
The Journal of biological chemistry 2020 Jun 5;295(23):7970-7980
ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation.
Lee TY, Cho IS, Bashyal N, Naya FJ, Tsai MJ, Yoon JS, Choi JM, Park CH, Kim SS, Suh-Kim H
Experimental neurobiology 2020 Jun 30;29(3):189-206
Experimental neurobiology 2020 Jun 30;29(3):189-206
Genomic deregulation of PRMT5 supports growth and stress tolerance in chronic lymphocytic leukemia.
Schnormeier AK, Pommerenke C, Kaufmann M, Drexler HG, Koeppel M
Scientific reports 2020 Jun 17;10(1):9775
Scientific reports 2020 Jun 17;10(1):9775
Argonaute binding within 3'-untranslated regions poorly predicts gene repression.
Chu Y, Kilikevicius A, Liu J, Johnson KC, Yokota S, Corey DR
Nucleic acids research 2020 Jul 27;48(13):7439-7453
Nucleic acids research 2020 Jul 27;48(13):7439-7453
The yeast cell wall protein Pry3 inhibits mating through highly conserved residues within the CAP domain.
Cottier S, Darwiche R, Meyenhofer F, Debelyy MO, Schneiter R
Biology open 2020 Jul 2;9(6)
Biology open 2020 Jul 2;9(6)
Yeast surface display of full-length human microtubule-associated protein tau.
Wang S, Cho YK
Biotechnology progress 2020 Jan;36(1):e2920
Biotechnology progress 2020 Jan;36(1):e2920
Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis.
Huangyang P, Li F, Lee P, Nissim I, Weljie AM, Mancuso A, Li B, Keith B, Yoon SS, Simon MC
Cell metabolism 2020 Jan 7;31(1):174-188.e7
Cell metabolism 2020 Jan 7;31(1):174-188.e7
Image-Based Analysis of Protein Stability.
Hickman KA, Hariharan S, De Melo J, Ylanko J, Lustig LC, Penn LZ, Andrews DW
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2020 Apr;97(4):363-377
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2020 Apr;97(4):363-377
A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans.
Manage KI, Rogers AK, Wallis DC, Uebel CJ, Anderson DC, Nguyen DAH, Arca K, Brown KC, Cordeiro Rodrigues RJ, de Albuquerque BF, Ketting RF, Montgomery TA, Phillips CM
eLife 2020 Apr 27;9
eLife 2020 Apr 27;9
Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027.
Peng Z, Simeon R, Mitchell SB, Zhang J, Feng H, Chen Z
mSphere 2019 Oct 2;4(5)
mSphere 2019 Oct 2;4(5)
Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.
Xie Y, Sun J, Han X, Turšić-Wunder A, Toh JDW, Hong W, Gao YG, Miao Y
Nature communications 2019 Nov 7;10(1):5078
Nature communications 2019 Nov 7;10(1):5078
Molecular architecture of the Bardet-Biedl syndrome protein 2-7-9 subcomplex.
Ludlam WG, Aoba T, Cuéllar J, Bueno-Carrasco MT, Makaju A, Moody JD, Franklin S, Valpuesta JM, Willardson BM
The Journal of biological chemistry 2019 Nov 1;294(44):16385-16399
The Journal of biological chemistry 2019 Nov 1;294(44):16385-16399
Engineering of anti-human interleukin-4 receptor alpha antibodies with potent antagonistic activity.
Kim JE, Jung K, Kim JA, Kim SH, Park HS, Kim YS
Scientific reports 2019 May 23;9(1):7772
Scientific reports 2019 May 23;9(1):7772
The LIM protein Ajuba recruits DBC1 and CBP/p300 to acetylate ERα and enhances ERα target gene expression in breast cancer cells.
Xu B, Li Q, Chen N, Zhu C, Meng Q, Ayyanathan K, Qian W, Jia H, Wang J, Ni P, Hou Z
Nucleic acids research 2019 Mar 18;47(5):2322-2335
Nucleic acids research 2019 Mar 18;47(5):2322-2335
Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli.
Kwak MS, Kim HS, Lkhamsuren K, Kim YH, Han MG, Shin JM, Park IH, Rhee WJ, Lee SK, Rhee SG, Shin JS
Redox biology 2019 Jun;24:101203
Redox biology 2019 Jun;24:101203
Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B.
Simeon R, Jiang M, Chamoun-Emanuelli AM, Yu H, Zhang Y, Meng R, Peng Z, Jakana J, Zhang J, Feng H, Chen Z
PLoS biology 2019 Jun;17(6):e3000311
PLoS biology 2019 Jun;17(6):e3000311
Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.
Sigoillot M, Overtus M, Grodecka M, Scholl D, Garcia-Pino A, Laeremans T, He L, Pardon E, Hildebrandt E, Urbatsch I, Steyaert J, Riordan JR, Govaerts C
Nature communications 2019 Jun 14;10(1):2636
Nature communications 2019 Jun 14;10(1):2636
The Circadian tau Mutation in Casein Kinase 1 Is Part of a Larger Domain That Can Be Mutated to Shorten Circadian Period.
Venkatesan A, Fan JY, Bouyain S, Price JL
International journal of molecular sciences 2019 Feb 14;20(4)
International journal of molecular sciences 2019 Feb 14;20(4)
Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication.
DeDiego ML, Nogales A, Martinez-Sobrido L, Topham DJ
mBio 2019 Aug 27;10(4)
mBio 2019 Aug 27;10(4)
Involvement of PDZ-SAP97 interactions in regulating AQP2 translocation in response to vasopressin in LLC-PK(1) cells.
Nooh MM, Kale A, Bahouth SW
American journal of physiology. Renal physiology 2019 Aug 1;317(2):F375-F387
American journal of physiology. Renal physiology 2019 Aug 1;317(2):F375-F387
The binding of Chp2's chromodomain to methylated H3K9 is essential for Chp2's role in heterochromatin assembly in fission yeast.
Maksimov V, Oya E, Tanaka M, Kawaguchi T, Hachisuka A, Ekwall K, Bjerling P, Nakayama JI
PloS one 2018;13(8):e0201101
PloS one 2018;13(8):e0201101
Structural basis for arginine glycosylation of host substrates by bacterial effector proteins.
Park JB, Kim YH, Yoo Y, Kim J, Jun SH, Cho JW, El Qaidi S, Walpole S, Monaco S, García-García AA, Wu M, Hays MP, Hurtado-Guerrero R, Angulo J, Hardwidge PR, Shin JS, Cho HS
Nature communications 2018 Oct 16;9(1):4283
Nature communications 2018 Oct 16;9(1):4283
A biosensor for MAPK-dependent Lin28 signaling.
Oldach LM, Gorshkov K, Mills WT 4th, Zhang J, Meffert MK
Molecular biology of the cell 2018 May 15;29(10):1157-1167
Molecular biology of the cell 2018 May 15;29(10):1157-1167
C8orf46 homolog encodes a novel protein Vexin that is required for neurogenesis in Xenopus laevis.
Moore KB, Logan MA, Aldiri I, Roberts JM, Steele M, Vetter ML
Developmental biology 2018 May 1;437(1):27-40
Developmental biology 2018 May 1;437(1):27-40
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function.
Zhao Y, Wu X, Li X, Jiang LL, Gui X, Liu Y, Sun Y, Zhu B, Piña-Crespo JC, Zhang M, Zhang N, Chen X, Bu G, An Z, Huang TY, Xu H
Neuron 2018 Mar 7;97(5):1023-1031.e7
Neuron 2018 Mar 7;97(5):1023-1031.e7
Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes.
Vitali DG, Käser S, Kolb A, Dimmer KS, Schneider A, Rapaport D
eLife 2018 Jun 20;7
eLife 2018 Jun 20;7
Molecular basis of titin exon exclusion by RBM20 and the novel titin splice regulator PTB4.
Dauksaite V, Gotthardt M
Nucleic acids research 2018 Jun 1;46(10):5227-5238
Nucleic acids research 2018 Jun 1;46(10):5227-5238
Bmp2 and Notch cooperate to pattern the embryonic endocardium.
Papoutsi T, Luna-Zurita L, Prados B, Zaffran S, de la Pompa JL
Development (Cambridge, England) 2018 Jul 2;145(13)
Development (Cambridge, England) 2018 Jul 2;145(13)
A biosensor-based framework to measure latent proteostasis capacity.
Wood RJ, Ormsby AR, Radwan M, Cox D, Sharma A, Vöpel T, Ebbinghaus S, Oliveberg M, Reid GE, Dickson A, Hatters DM
Nature communications 2018 Jan 18;9(1):287
Nature communications 2018 Jan 18;9(1):287
Interaction between ROR1 and MuSK activation complex in myogenic cells.
Karvonen H, Summala K, Niininen W, Barker HR, Ungureanu D
FEBS letters 2018 Feb;592(3):434-445
FEBS letters 2018 Feb;592(3):434-445
Inflachromene inhibits autophagy through modulation of Beclin 1 activity.
Kim YH, Kwak MS, Shin JM, Hayuningtyas RA, Choi JE, Shin JS
Journal of cell science 2018 Feb 20;131(4)
Journal of cell science 2018 Feb 20;131(4)
SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity.
Orosa B, Yates G, Verma V, Srivastava AK, Srivastava M, Campanaro A, De Vega D, Fernandes A, Zhang C, Lee J, Bennett MJ, Sadanandom A
Nature communications 2018 Dec 5;9(1):5185
Nature communications 2018 Dec 5;9(1):5185
High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles.
Longatti A, Schindler C, Collinson A, Jenkinson L, Matthews C, Fitzpatrick L, Blundy M, Minter R, Vaughan T, Shaw M, Tigue N
Nanoscale 2018 Aug 7;10(29):14230-14244
Nanoscale 2018 Aug 7;10(29):14230-14244
Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling.
Kourtidis A, Necela B, Lin WH, Lu R, Feathers RW, Asmann YW, Thompson EA, Anastasiadis PZ
The Journal of cell biology 2017 Oct 2;216(10):3073-3085
The Journal of cell biology 2017 Oct 2;216(10):3073-3085
Charting organellar importomes by quantitative mass spectrometry.
Peikert CD, Mani J, Morgenstern M, Käser S, Knapp B, Wenger C, Harsman A, Oeljeklaus S, Schneider A, Warscheid B
Nature communications 2017 May 9;8:15272
Nature communications 2017 May 9;8:15272
Clinical-scale expansion of CD34(+) cord blood cells amplifies committed progenitors and rapid scid repopulation cells.
Casamayor-Genescà A, Pla A, Oliver-Vila I, Pujals-Fonts N, Marín-Gallén S, Caminal M, Pujol-Autonell I, Carrascal J, Vives-Pi M, Garcia J, Vives J
New biotechnology 2017 Mar 25;35:19-29
New biotechnology 2017 Mar 25;35:19-29
Dentin sialoprotein facilitates dental mesenchymal cell differentiation and dentin formation.
Li W, Chen L, Chen Z, Wu L, Feng J, Wang F, Shoff L, Li X, Donly KJ, MacDougall M, Chen S
Scientific reports 2017 Mar 22;7(1):300
Scientific reports 2017 Mar 22;7(1):300
An engineered protein antagonist of K-Ras/B-Raf interaction.
Kauke MJ, Traxlmayr MW, Parker JA, Kiefer JD, Knihtila R, McGee J, Verdine G, Mattos C, Wittrup KD
Scientific reports 2017 Jul 19;7(1):5831
Scientific reports 2017 Jul 19;7(1):5831
Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei.
Mani J, Rout S, Desy S, Schneider A
Scientific reports 2017 Jan 17;7:40738
Scientific reports 2017 Jan 17;7:40738
Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1.
Dubey R, Staker BL, Foe IT, Bogyo M, Myler PJ, Ngô HM, Gubbels MJ
Molecular microbiology 2017 Feb;103(4):618-634
Molecular microbiology 2017 Feb;103(4):618-634
Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease.
Hubmacher D, Schneider M, Berardinelli SJ, Takeuchi H, Willard B, Reinhardt DP, Haltiwanger RS, Apte SS
Scientific reports 2017 Feb 8;7:41871
Scientific reports 2017 Feb 8;7:41871
Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei.
Käser S, Willemin M, Schnarwiler F, Schimanski B, Poveda-Huertes D, Oeljeklaus S, Haenni B, Zuber B, Warscheid B, Meisinger C, Schneider A
PLoS pathogens 2017 Dec;13(12):e1006808
PLoS pathogens 2017 Dec;13(12):e1006808
A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase.
Wenger C, Oeljeklaus S, Warscheid B, Schneider A, Harsman A
PLoS pathogens 2017 Aug;13(8):e1006550
PLoS pathogens 2017 Aug;13(8):e1006550
PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.
Kourtidis A, Anastasiadis PZ
Cell cycle (Georgetown, Tex.) 2016;15(4):498-505
Cell cycle (Georgetown, Tex.) 2016;15(4):498-505
Drosophila Mitf regulates the V-ATPase and the lysosomal-autophagic pathway.
Bouché V, Espinosa AP, Leone L, Sardiello M, Ballabio A, Botas J
Autophagy 2016;12(3):484-98
Autophagy 2016;12(3):484-98
Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism.
Wang Y, Li Y, Hu G, Huang X, Rao H, Xiong X, Luo Z, Lu Q, Luo S
Cellular signalling 2016 Sep;28(9):1304-1313
Cellular signalling 2016 Sep;28(9):1304-1313
Impaired ADAMTS9 secretion: A potential mechanism for eye defects in Peters Plus Syndrome.
Dubail J, Vasudevan D, Wang LW, Earp SE, Jenkins MW, Haltiwanger RS, Apte SS
Scientific reports 2016 Sep 30;6:33974
Scientific reports 2016 Sep 30;6:33974
The NPC2 protein: A novel dog allergen.
Khurana T, Newman-Lindsay S, Young PR, Slater JE
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2016 May;116(5):440-446.e2
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2016 May;116(5):440-446.e2
cMyc-p53 feedback mechanism regulates the dynamics of T lymphocytes in the immune response.
Madapura HS, Salamon D, Wiman KG, Lain S, Klein E, Nagy N
Cell cycle (Georgetown, Tex.) 2016 May 2;15(9):1267-75
Cell cycle (Georgetown, Tex.) 2016 May 2;15(9):1267-75
Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.
Kanemori Y, Koga Y, Sudo M, Kang W, Kashiwabara S, Ikawa M, Hasuwa H, Nagashima K, Ishikawa Y, Ogonuki N, Ogura A, Baba T
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 28;113(26):E3696-705
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 28;113(26):E3696-705
Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion.
Boateng LR, Bennin D, De Oliveira S, Huttenlocher A
The Journal of biological chemistry 2016 Jul 1;291(27):13987-13998
The Journal of biological chemistry 2016 Jul 1;291(27):13987-13998
The LIM protein Ajuba promotes adipogenesis by enhancing PPARγ and p300/CBP interaction.
Li Q, Peng H, Fan H, Zou X, Liu Q, Zhang Y, Xu H, Chu Y, Wang C, Ayyanathan K, Rauscher FJ, Zhang K, Hou Z
Cell death and differentiation 2016 Jan;23(1):158-68
Cell death and differentiation 2016 Jan;23(1):158-68
The levels of epithelial anchor proteins β-catenin and zona occludens-1 are altered by E7 of human papillomaviruses 5 and 8.
Heuser S, Hufbauer M, Marx B, Tok A, Majewski S, Pfister H, Akgül B
The Journal of general virology 2016 Feb;97(2):463-472
The Journal of general virology 2016 Feb;97(2):463-472
The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins.
Harsman A, Oeljeklaus S, Wenger C, Huot JL, Warscheid B, Schneider A
Nature communications 2016 Dec 19;7:13707
Nature communications 2016 Dec 19;7:13707
Outer membrane protein functions as integrator of protein import and DNA inheritance in mitochondria.
Käser S, Oeljeklaus S, Týč J, Vaughan S, Warscheid B, Schneider A
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 2;113(31):E4467-75
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 2;113(31):E4467-75
The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein.
Shi C, Huang X, Zhang B, Zhu D, Luo H, Lu Q, Xiong WC, Mei L, Luo S
PloS one 2015;10(9):e0138936
PloS one 2015;10(9):e0138936
Delineating the Tes Interaction Site in Zyxin and Studying Cellular Effects of Its Disruption.
Hadzic E, Catillon M, Halavatyi A, Medves S, Van Troys M, Moes M, Baird MA, Davidson MW, Schaffner-Reckinger E, Ampe C, Friederich E
PloS one 2015;10(10):e0140511
PloS one 2015;10(10):e0140511
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
Asghar A, Lajeunesse A, Dulla K, Combes G, Thebault P, Nigg EA, Elowe S
Nature communications 2015 Sep 24;6:8364
Nature communications 2015 Sep 24;6:8364
Ajuba Preferentially Binds LXRα/RXRγ Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.
Fan H, Dong W, Li Q, Zou X, Zhang Y, Wang J, Li S, Liu W, Dong Y, Sun H, Hou Z
Molecular endocrinology (Baltimore, Md.) 2015 Nov;29(11):1608-18
Molecular endocrinology (Baltimore, Md.) 2015 Nov;29(11):1608-18
Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy.
Desantis A, Bruno T, Catena V, De Nicola F, Goeman F, Iezzi S, Sorino C, Ponzoni M, Bossi G, Federico V, La Rosa F, Ricciardi MR, Lesma E, De Meo PD, Castrignanò T, Petrucci MT, Pisani F, Chesi M, Bergsagel PL, Floridi A, Tonon G, Passananti C, Blandino G, Fanciulli M
The EMBO journal 2015 May 5;34(9):1214-30
The EMBO journal 2015 May 5;34(9):1214-30
Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances.
Mani J, Desy S, Niemann M, Chanfon A, Oeljeklaus S, Pusnik M, Schmidt O, Gerbeth C, Meisinger C, Warscheid B, Schneider A
Nature communications 2015 Mar 26;6:6646
Nature communications 2015 Mar 26;6:6646
The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units.
Merrikh CN, Brewer BJ, Merrikh H
PLoS genetics 2015 Jun;11(6):e1005289
PLoS genetics 2015 Jun;11(6):e1005289
Maternal dazap2 Regulates Germ Granules by Counteracting Dynein in Zebrafish Primordial Germ Cells.
Forbes MM, Rothhämel S, Jenny A, Marlow FL
Cell reports 2015 Jul 7;12(1):49-57
Cell reports 2015 Jul 7;12(1):49-57
Structures of the Gβ-CCT and PhLP1-Gβ-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγ dimer assembly.
Plimpton RL, Cuéllar J, Lai CW, Aoba T, Makaju A, Franklin S, Mathis AD, Prince JT, Carrascosa JL, Valpuesta JM, Willardson BM
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 24;112(8):2413-8
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 24;112(8):2413-8
Novel components of the Toxoplasma inner membrane complex revealed by BioID.
Chen AL, Kim EW, Toh JY, Vashisht AA, Rashoff AQ, Van C, Huang AS, Moon AS, Bell HN, Bentolila LA, Wohlschlegel JA, Bradley PJ
mBio 2015 Feb 17;6(1):e02357-14
mBio 2015 Feb 17;6(1):e02357-14
Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG.
Fischer N, Elson G, Magistrelli G, Dheilly E, Fouque N, Laurendon A, Gueneau F, Ravn U, Depoisier JF, Moine V, Raimondi S, Malinge P, Di Grazia L, Rousseau F, Poitevin Y, Calloud S, Cayatte PA, Alcoz M, Pontini G, Fagète S, Broyer L, Corbier M, Schrag D, Didelot G, Bosson N, Costes N, Cons L, Buatois V, Johnson Z, Ferlin W, Masternak K, Kosco-Vilbois M
Nature communications 2015 Feb 12;6:6113
Nature communications 2015 Feb 12;6:6113
The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling.
Hunt LC, Xu B, Finkelstein D, Fan Y, Carroll PA, Cheng PF, Eisenman RN, Demontis F
Genes & development 2015 Dec 1;29(23):2475-89
Genes & development 2015 Dec 1;29(23):2475-89
RON5 is critical for organization and function of the Toxoplasma moving junction complex.
Beck JR, Chen AL, Kim EW, Bradley PJ
PLoS pathogens 2014 Mar;10(3):e1004025
PLoS pathogens 2014 Mar;10(3):e1004025
Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells.
Xiong X, Wang Y, Liu C, Lu Q, Liu T, Chen G, Rao H, Luo S
Experimental cell research 2014 Aug 1;326(1):78-89
Experimental cell research 2014 Aug 1;326(1):78-89
Role of phosphatidylinositol 4,5-bisphosphate in regulating EHD2 plasma membrane localization.
Simone LC, Caplan S, Naslavsky N
PloS one 2013;8(9):e74519
PloS one 2013;8(9):e74519
The human minor histocompatibility antigen 1 is a RhoGAP.
de Kreuk BJ, Schaefer A, Anthony EC, Tol S, Fernandez-Borja M, Geerts D, Pool J, Hambach L, Goulmy E, Hordijk PL
PloS one 2013;8(9):e73962
PloS one 2013;8(9):e73962
CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire.
Wu S, Zhu W, Nhan T, Toth JI, Petroski MD, Wolf DA
Nature communications 2013;4:1642
Nature communications 2013;4:1642
Determinants at the N- and C-termini of Gα12 required for activation of Rho-mediated signaling.
Ritchie BJ, Smolski WC, Montgomery ER, Fisher ES, Choi TY, Olson CM, Foster LA, Meigs TE
Journal of molecular signaling 2013 Mar 25;8(1):3
Journal of molecular signaling 2013 Mar 25;8(1):3
Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells.
Shevchuk Z, Yurchenko MY, Darekar SD, Holodnuka-Kholodnyuk I, Kashuba VI, Kashuba EV
Acta naturae 2013 Jan;5(1):85-9
Acta naturae 2013 Jan;5(1):85-9
Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation.
Chandra S, Priyadarshini R, Madhavan V, Tikoo S, Hussain M, Mudgal R, Modi P, Srivastava V, Sengupta S
Journal of cell science 2013 Aug 15;126(Pt 16):3782-95
Journal of cell science 2013 Aug 15;126(Pt 16):3782-95
The ATM substrate KAP1 controls DNA repair in heterochromatin: regulation by HP1 proteins and serine 473/824 phosphorylation.
White D, Rafalska-Metcalf IU, Ivanov AV, Corsinotti A, Peng H, Lee SC, Trono D, Janicki SM, Rauscher FJ 3rd
Molecular cancer research : MCR 2012 Mar;10(3):401-14
Molecular cancer research : MCR 2012 Mar;10(3):401-14
Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts.
Nethe M, de Kreuk BJ, Tauriello DV, Anthony EC, Snoek B, Stumpel T, Salinas PC, Maurice MM, Geerts D, Deelder AM, Hensbergen PJ, Hordijk PL
Journal of cell science 2012 Jul 15;125(Pt 14):3430-42
Journal of cell science 2012 Jul 15;125(Pt 14):3430-42
The F-BAR protein PACSIN2 regulates epidermal growth factor receptor internalization.
de Kreuk BJ, Anthony EC, Geerts D, Hordijk PL
The Journal of biological chemistry 2012 Dec 21;287(52):43438-53
The Journal of biological chemistry 2012 Dec 21;287(52):43438-53
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
Huang YS, Chiang NY, Hu CH, Hsiao CC, Cheng KF, Tsai WP, Yona S, Stacey M, Gordon S, Chang GW, Lin HH
Molecular and cellular biology 2012 Apr;32(8):1408-20
Molecular and cellular biology 2012 Apr;32(8):1408-20
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Challagundla KB, Sun XX, Zhang X, DeVine T, Zhang Q, Sears RC, Dai MS
Molecular and cellular biology 2011 Oct;31(19):4007-21
Molecular and cellular biology 2011 Oct;31(19):4007-21
Interactome mapping suggests new mechanistic details underlying Alzheimer's disease.
Soler-López M, Zanzoni A, Lluís R, Stelzl U, Aloy P
Genome research 2011 Mar;21(3):364-76
Genome research 2011 Mar;21(3):364-76
Array-based comparative genomic hybridization identifies CDK4 and FOXM1 alterations as independent predictors of survival in malignant peripheral nerve sheath tumor.
Yu J, Deshmukh H, Payton JE, Dunham C, Scheithauer BW, Tihan T, Prayson RA, Guha A, Bridge JA, Ferner RE, Lindberg GM, Gutmann RJ, Emnett RJ, Salavaggione L, Gutmann DH, Nagarajan R, Watson MA, Perry A
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Apr 1;17(7):1924-34
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Apr 1;17(7):1924-34
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development 2010 Sep 1;5:24
Neural development 2010 Sep 1;5:24
ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
Koo BH, Coe DM, Dixon LJ, Somerville RP, Nelson CM, Wang LW, Young ME, Lindner DJ, Apte SS
The American journal of pathology 2010 Mar;176(3):1494-504
The American journal of pathology 2010 Mar;176(3):1494-504
Aurora-A phosphorylates, activates, and relocalizes the small GTPase RalA.
Lim KH, Brady DC, Kashatus DF, Ancrile BB, Der CJ, Cox AD, Counter CM
Molecular and cellular biology 2010 Jan;30(2):508-23
Molecular and cellular biology 2010 Jan;30(2):508-23
Ribosomal protein L11 associates with c-Myc at 5 S rRNA and tRNA genes and regulates their expression.
Dai MS, Sun XX, Lu H
The Journal of biological chemistry 2010 Apr 23;285(17):12587-94
The Journal of biological chemistry 2010 Apr 23;285(17):12587-94
Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis.
Hood FE, Royle SJ
Journal of cell science 2009 Jul 1;122(Pt 13):2185-90
Journal of cell science 2009 Jul 1;122(Pt 13):2185-90
Classic and desmoplastic medulloblastoma: complete case reports and characterizations of two new cell lines.
Holthouse DJ, Dallas PB, Ford J, Fabian V, Murch AR, Watson M, Wong G, Bertram C, Egli S, Baker DL, Kees UR
Neuropathology : official journal of the Japanese Society of Neuropathology 2009 Aug;29(4):398-409
Neuropathology : official journal of the Japanese Society of Neuropathology 2009 Aug;29(4):398-409
Regulatory mechanism of matrix metalloprotease-2 enzymatic activity by factor Xa and thrombin.
Koo BH, Park MY, Jeon OH, Kim DS
The Journal of biological chemistry 2009 Aug 28;284(35):23375-85
The Journal of biological chemistry 2009 Aug 28;284(35):23375-85
An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization.
Huot ME, Brown CM, Lamarche-Vane N, Richard S
Molecular and cellular biology 2009 Apr;29(7):1933-43
Molecular and cellular biology 2009 Apr;29(7):1933-43
Immunohistochemical analysis of the progression of flat and papillary preneoplastic lesions in intrahepatic cholangiocarcinogenesis in hepatolithiasis.
Itatsu K, Zen Y, Ohira S, Ishikawa A, Sato Y, Harada K, Ikeda H, Sasaki M, Nimura Y, Nakanuma Y
Liver international : official journal of the International Association for the Study of the Liver 2007 Nov;27(9):1174-84
Liver international : official journal of the International Association for the Study of the Liver 2007 Nov;27(9):1174-84
Regulation of ADAMTS9 secretion and enzymatic activity by its propeptide.
Koo BH, Longpré JM, Somerville RP, Alexander JP, Leduc R, Apte SS
The Journal of biological chemistry 2007 Jun 1;282(22):16146-54
The Journal of biological chemistry 2007 Jun 1;282(22):16146-54
Analysis of a Shc family adaptor protein, ShcD/Shc4, that associates with muscle-specific kinase.
Jones N, Hardy WR, Friese MB, Jorgensen C, Smith MJ, Woody NM, Burden SJ, Pawson T
Molecular and cellular biology 2007 Jul;27(13):4759-73
Molecular and cellular biology 2007 Jul;27(13):4759-73
The prolyl isomerase Pin1 affects Che-1 stability in response to apoptotic DNA damage.
De Nicola F, Bruno T, Iezzi S, Di Padova M, Floridi A, Passananti C, Del Sal G, Fanciulli M
The Journal of biological chemistry 2007 Jul 6;282(27):19685-91
The Journal of biological chemistry 2007 Jul 6;282(27):19685-91
Kinetic mechanism of the fastest motor protein, Chara myosin.
Ito K, Ikebe M, Kashiyama T, Mogami T, Kon T, Yamamoto K
The Journal of biological chemistry 2007 Jul 6;282(27):19534-45
The Journal of biological chemistry 2007 Jul 6;282(27):19534-45
Mutations in PDLIM3 and MYOZ1 encoding myocyte Z line proteins are infrequently found in idiopathic dilated cardiomyopathy.
Arola AM, Sanchez X, Murphy RT, Hasle E, Li H, Elliott PM, McKenna WJ, Towbin JA, Bowles NE
Molecular genetics and metabolism 2007 Apr;90(4):435-40
Molecular genetics and metabolism 2007 Apr;90(4):435-40
The binding of DYNLL2 to myosin Va requires alternatively spliced exon B and stabilizes a portion of the myosin's coiled-coil domain.
Wagner W, Fodor E, Ginsburg A, Hammer JA 3rd
Biochemistry 2006 Sep 26;45(38):11564-77
Biochemistry 2006 Sep 26;45(38):11564-77
Activation of transferrin receptor 1 by c-Myc enhances cellular proliferation and tumorigenesis.
O'Donnell KA, Yu D, Zeller KI, Kim JW, Racke F, Thomas-Tikhonenko A, Dang CV
Molecular and cellular biology 2006 Mar;26(6):2373-86
Molecular and cellular biology 2006 Mar;26(6):2373-86
AP-1 differentially expressed proteins Krp1 and fibronectin cooperatively enhance Rho-ROCK-independent mesenchymal invasion by altering the function, localization, and activity of nondifferentially expressed proteins.
Spence HJ, McGarry L, Chew CS, Carragher NO, Scott-Carragher LA, Yuan Z, Croft DR, Olson MF, Frame M, Ozanne BW
Molecular and cellular biology 2006 Feb;26(4):1480-95
Molecular and cellular biology 2006 Feb;26(4):1480-95
Characterization of the human GARP (Golgi associated retrograde protein) complex.
Liewen H, Meinhold-Heerlein I, Oliveira V, Schwarzenbacher R, Luo G, Wadle A, Jung M, Pfreundschuh M, Stenner-Liewen F
Experimental cell research 2005 May 15;306(1):24-34
Experimental cell research 2005 May 15;306(1):24-34
Function and regulation of Tumbleweed (RacGAP50C) in neuroblast proliferation and neuronal morphogenesis.
Goldstein AY, Jan YN, Luo L
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 8;102(10):3834-9
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 8;102(10):3834-9
Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE.
Honda A, Al-Awar OS, Hay JC, Donaldson JG
The Journal of cell biology 2005 Mar 28;168(7):1039-51
The Journal of cell biology 2005 Mar 28;168(7):1039-51
Functional properties of the p33 and p55 domains of the Helicobacter pylori vacuolating cytotoxin.
Torres VJ, Ivie SE, McClain MS, Cover TL
The Journal of biological chemistry 2005 Jun 3;280(22):21107-14
The Journal of biological chemistry 2005 Jun 3;280(22):21107-14
Akt phosphorylates Tal1 oncoprotein and inhibits its repressor activity.
Palamarchuk A, Efanov A, Maximov V, Aqeilan RI, Croce CM, Pekarsky Y
Cancer research 2005 Jun 1;65(11):4515-9
Cancer research 2005 Jun 1;65(11):4515-9
Proteolytic maturation and activation of autotaxin (NPP2), a secreted metastasis-enhancing lysophospholipase D.
Jansen S, Stefan C, Creemers JW, Waelkens E, Van Eynde A, Stalmans W, Bollen M
Journal of cell science 2005 Jul 15;118(Pt 14):3081-9
Journal of cell science 2005 Jul 15;118(Pt 14):3081-9
Trapping prion protein in the endoplasmic reticulum impairs PrPC maturation and prevents PrPSc accumulation.
Cardinale A, Filesi I, Vetrugno V, Pocchiari M, Sy MS, Biocca S
The Journal of biological chemistry 2005 Jan 7;280(1):685-94
The Journal of biological chemistry 2005 Jan 7;280(1):685-94
Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1.
Zhang J, Wang Y, Zhou Y, Cao Z, Huang P, Lu B
FEBS letters 2005 Jan 17;579(2):559-66
FEBS letters 2005 Jan 17;579(2):559-66
Distinct roles of EGF repeats for the Notch signaling system.
Sakamoto K, Chao WS, Katsube K, Yamaguchi A
Experimental cell research 2005 Jan 15;302(2):281-91
Experimental cell research 2005 Jan 15;302(2):281-91
Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin.
Soosairajah J, Maiti S, Wiggan O, Sarmiere P, Moussi N, Sarcevic B, Sampath R, Bamburg JR, Bernard O
The EMBO journal 2005 Feb 9;24(3):473-86
The EMBO journal 2005 Feb 9;24(3):473-86
Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration.
Pyagay P, Heroult M, Wang Q, Lehnert W, Belden J, Liaw L, Friesel RE, Lindner V
Circulation research 2005 Feb 4;96(2):261-8
Circulation research 2005 Feb 4;96(2):261-8
HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.
Iwata A, Riley BE, Johnston JA, Kopito RR
The Journal of biological chemistry 2005 Dec 2;280(48):40282-92
The Journal of biological chemistry 2005 Dec 2;280(48):40282-92
WW domain-containing proteins, WWOX and YAP, compete for interaction with ErbB-4 and modulate its transcriptional function.
Aqeilan RI, Donati V, Palamarchuk A, Trapasso F, Kaou M, Pekarsky Y, Sudol M, Croce CM
Cancer research 2005 Aug 1;65(15):6764-72
Cancer research 2005 Aug 1;65(15):6764-72
A role for Ubc9 in tumorigenesis.
Mo YY, Yu Y, Theodosiou E, Ee PL, Beck WT
Oncogene 2005 Apr 14;24(16):2677-83
Oncogene 2005 Apr 14;24(16):2677-83
Isoforms of the polarity protein par6 have distinct functions.
Gao L, Macara IG
The Journal of biological chemistry 2004 Oct 1;279(40):41557-62
The Journal of biological chemistry 2004 Oct 1;279(40):41557-62
Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle.
Kaplan DD, Meigs TE, Kelly P, Casey PJ
The Journal of biological chemistry 2004 Mar 19;279(12):10829-32
The Journal of biological chemistry 2004 Mar 19;279(12):10829-32
A homologue of Cdk8 is required for spore cell differentiation in Dictyostelium.
Lin HH, Khosla M, Huang HJ, Hsu DW, Michaelis C, Weeks G, Pears C
Developmental biology 2004 Jul 1;271(1):49-58
Developmental biology 2004 Jul 1;271(1):49-58
Expression of c-erbB2, p53, Bcl-2, Bax, c-myc and Ki-67 in apocrine metaplasia and apocrine change within sclerosing adenosis of the breast.
Selim AG, El-Ayat G, Wells CA
Virchows Archiv : an international journal of pathology 2002 Nov;441(5):449-55
Virchows Archiv : an international journal of pathology 2002 Nov;441(5):449-55
Transendothelial migration of colon carcinoma cells requires expression of E-selectin by endothelial cells and activation of stress-activated protein kinase-2 (SAPK2/p38) in the tumor cells.
Laferriere J, Houle F, Taher MM, Valerie K, Huot J
The Journal of biological chemistry 2001 Sep 7;276(36):33762-72
The Journal of biological chemistry 2001 Sep 7;276(36):33762-72
Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic.
Brown FD, Rozelle AL, Yin HL, Balla T, Donaldson JG
The Journal of cell biology 2001 Sep 3;154(5):1007-17
The Journal of cell biology 2001 Sep 3;154(5):1007-17
Involvement of phospholipase D in sphingosine 1-phosphate-induced activation of phosphatidylinositol 3-kinase and Akt in Chinese hamster ovary cells overexpressing EDG3.
Banno Y, Takuwa Y, Akao Y, Okamoto H, Osawa Y, Naganawa T, Nakashima S, Suh PG, Nozawa Y
The Journal of biological chemistry 2001 Sep 21;276(38):35622-8
The Journal of biological chemistry 2001 Sep 21;276(38):35622-8
Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin.
Hilairet S, Bélanger C, Bertrand J, Laperrière A, Foord SM, Bouvier M
The Journal of biological chemistry 2001 Nov 9;276(45):42182-90
The Journal of biological chemistry 2001 Nov 9;276(45):42182-90
PYK2 as a mediator of endothelin-1/G alpha 11 signaling to GLUT4 glucose transporters.
Park JG, Bose A, Leszyk J, Czech MP
The Journal of biological chemistry 2001 Dec 21;276(51):47751-4
The Journal of biological chemistry 2001 Dec 21;276(51):47751-4
Leucine zipper-mediated homodimerization of the p21-activated kinase-interacting factor, beta Pix. Implication for a role in cytoskeletal reorganization.
Kim S, Lee SH, Park D
The Journal of biological chemistry 2001 Apr 6;276(14):10581-4
The Journal of biological chemistry 2001 Apr 6;276(14):10581-4
p59fyn tyrosine kinase associates with multiple T-cell receptor subunits through its unique amino-terminal domain.
Timson Gauen LK, Kong AN, Samelson LE, Shaw AS
Molecular and cellular biology 1992 Dec;12(12):5438-46
Molecular and cellular biology 1992 Dec;12(12):5438-46
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Evan GI, Lewis GK, Ramsay G, Bishop JM
Molecular and cellular biology 1985 Dec;5(12):3610-6
Molecular and cellular biology 1985 Dec;5(12):3610-6
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Evan GI, Lewis GK, Ramsay G, Bishop JM
Molecular and cellular biology 1985 Dec;5(12):3610-6
Molecular and cellular biology 1985 Dec;5(12):3610-6
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Evan GI, Lewis GK, Ramsay G, Bishop JM
Molecular and cellular biology 1985 Dec;5(12):3610-6
Molecular and cellular biology 1985 Dec;5(12):3610-6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of 293 cell lysate expressing a Myc-tagged protein using Mouse anti-c-Myc Monoclonal Antibody (Product # 13-2500)
- Submitted by
- Invitrogen Antibodies (provider)
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- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), HEK 293 (Lane 2), A-375 (Lane 3), HeLa (Lane 4), SH-SY5Y (Lane 5), U-87MG (Lane 6) and K562 (Lane 7). The blots were probed with Anti-c-Myc Epitope Tag Mouse Monoclonal Antibody (Product # 13-2500, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A ~ 50 kDa band corresponding to c-Myc was observed across cell lines tested except U-87 MG. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
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- Western blot was performed using Anti-Myc Tag Polyclonal Antibody (Product # 13-2500) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), His-H3-Myc, 40 µg (Lane 3), His-H3-Myc, 20 µg (Lane 4), His-H3-Myc, 10 µg (Lane 5), Myc-p65-V5, 40 µg (Lane 6), p65-HA, 40 µg (Lane 7) and 25 ng of Positope (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). A ~65 kDa band corresponding to Myc-p65-V5 and ~17 kDa band corresponding to His-H3-Myc was observed in HEK293E transfected lysates on probing with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product #A28177, 1:4000 dilution). Positope (Product # R90050) is a 53 kDa recombinant protein consisting of multiple epitope tags but lacks HA tag, which has been used as a positive control for c-Myc tag detection. No cross-reactivity was seen with HA-tagged p65 expressing lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Myc tag was performed using 70% confluent His-H3-Myc transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myc Tag Monoclonal Antibody (Product # 13-2500) at 2 µg/mL and Histone H3 antibody (Product # 711055) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight, and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32728) and Goat anti-Rabbit IgG Alexa Fluor Plus 488 (Product # A32731) respectively at a dilution of 1:2000 for 45 minutes at room temperature. Panel a (Nuclei: Red) represents the Myc tag. Panel b (Nuclei: Green) represents Histone 3. Panel c (Nuclei: Blue) represents ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel d represents the merged image showing the co-localization of nuclear signals in transfected cells. Panel e represents un-transfected HEK-293 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry analysis of c-Myc showing staining in the nucleus of paraffin-embedded human lung squamous carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- c-Myc Monoclonal Antibody (9E10) (Product # 13-2500) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- HEK293E cells were either untransfected or transfected with an Myc-Tagged Human H3 construct. Chromatin Immunoprecipitation (ChIP) was performed using target H3 antibody (Product # 711055) or c-Myc Monoclonal Antibody (9E10) (Product # 13-2500, 5 µg) on sheared chromatin from untransfected and myc tag transfected HEK293E cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control for target H3 antibody and Normal Mouse IgG for Myc Tag antibody. The purified DNA was analyzed by qPCR using primers binding to RPL30, ALDOA, PABPC1, cFOS and MYOD. Data is presented as fold enrichment of the antibody signal in untransfected and Myc tagged Human H3 construct transfected cells versus the negative control IgG using the comparative CT method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation (IP) studies of Myc tag were performed with c-Myc Monoclonal Antibody (9E10) (Product # 13-2500) (Panel I, II) or NFkB p65 Monoclonal Antibody (Product # 33-9900) (Panel III, IV) using the Dynabeads® Protein G Immunoprecipitation Kit (Product # 10007D). Lane 1,2: Total cell extract of HEK-293E untransfected (UT) and transfected (T) with Myc-tagged NFkB p65 construct (7% of input) Lane 3,4: IP of UT and T HEK-293E lysate using Isotype-matched Mouse IgG (Product # 31903) Lane 5,6: IP of UT and T HEK-293E lysate with 5 µg of c-Myc Monoclonal Antibody (9E10) (Product # 13-2500) (Panel I, II) (Panel I, II) or NFkB p65 Monoclonal Antibody (Product # 33-9900) (Panel III, IV) IP was analyzed using western blot. Myc-tagged NFkB p65 was detected at ~65kDa by probing the blot with NFkB p65 Monoclonal Antibody (Product # 33-9900) (0.5 µg/mL dilution) (Panel I, IV) or c-Myc Monoclonal Antibody (9E10) (Product # 13-2500) (1 µg/mL dilution) (Panel II, III) and detected by chemiluminescence with Recombinant Protein G-HRP (Product # 101223) (1:500 dilution) using the iBright FL 1500 (Product # A44241). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
