- 13-2511 - Invitrogen Antibodies MYC antibody

Submitted references Development of CAR-T Cell Persistence in Adoptive Immunotherapy of Solid Tumors.

Fan J, Das JK, Xiong X, Chen H, Song J
Frontiers in oncology 2020;10:574860

Differential Rac1 signalling by guanine nucleotide exchange factors implicates FLII in regulating Rac1-driven cell migration.

Marei H, Carpy A, Woroniuk A, Vennin C, White G, Timpson P, Macek B, Malliri A
Nature communications 2016 Feb 18;7:10664

Proton-sensing G-protein-coupled receptors.

Ludwig MG, Vanek M, Guerini D, Gasser JA, Jones CE, Junker U, Hofstetter H, Wolf RM, Seuwen K
Nature 2003 Sep 4;425(6953):93-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Myc proto-oncogene protein was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Myc Tag Monoclonal Antibody (Product # 13-2511) at 2 µg/mL in 0.1% BSA and Histone H3 antibody (Product # 711055) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight, and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product #A32733) at a dilution of 1:2000 for 45 minutes at room temperature. Panel a (Nuclei: Green) represents Myc tag. Panel b (Nuclei: Red) represents Histone H3. Panel c (Nuclei: Blue) represents ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel d represents the merged image showing the co-localization of nuclear signals in transfected cells. Panel e represents un-transfected HEK-293 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Activation of Rac1 by Tiam1 or P-Rex1 induces differential effects in A431 cells. ( a ) Representative phase-contrast images of A431 cells treated with ethanol (-dox) or 1 mug ml -1 doxycycline (+dox) for 24 h to induce expression of indicated GEF constructs. Scale bar, 100 mum. ( b ) Quantification of GEF-induced cellular phenotypes depicted in a . Graph represents per cent cells with the indicated morphology from a total of 150 cells per condition from three independent experiments. ( c ) Representative immunofluorescence images of +dox treated A431 cells fixed in 4% formaldehyde. Phalloidin staining was used to visualize the actin cytoskeleton and antibodies against HA-tagged Tiam1 WT and GEF* or Myc-tagged P-Rex1 WT and GEF* to detect the expression of the respective GEFs upon dox induction. DAPI was used to stain the nuclei. Scale bar, 100 mum. ( d ) Representative phase-contrast images of +dox treated A431 cells either left untreated (-EGF) or treated with 100 ng ml -1 epidermal growth factor (+EGF) for the indicated times following serum starvation for 18-24 h. Yellow boxes are used to highlight the differential effects of Tiam1 WT and P-Rex1 WT on cell scattering at indicated time points. Scale bar, 100 mum. ( e ) Quantification of cell scattering of+dox treated A431 cells described in d . Graph represents per cent of scattered or unscattered colonies from a total of 50 colonies per condition from three independent experiments. ( f ) Lysates from A431 cells f
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Carcinoembryonic antigen (CEA) chimeric antigen receptor T (CAR-T) cells overexpressing Bcl-xL prominently accumulated in tumor tissues. C57BL/6 mice were adoptively transferred with CEA CAR-T cells expressing with or without Bcl-xL or control cells one week after the mice were s.c. injected in the flank region with MC-38 or MC-32 tumor cells. On day 21 to 22 after tumor challenge, tumor tissues were examined for tumor-reactive T cell infiltration. (A) H&E staining (scale bars: 25 mum). (B) Immunohistological staining (scale bars: 50 mum). Myc + CEA CAR-T cells (green) infiltrated in tumor tissues (the dark background). (C) Single-cell suspensions from tumor tissues were analyzed for expression of CD8 + and Myc + T cells by flow cytometry, after gating on the CD8 + population. Data are representative of three independent experiments.
Conjugate: Green dye

13-2511

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