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- Antigen structure
- References [3]
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- Product number
- MA1-980-HRP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Myc Monoclonal Antibody (9E10), HRP
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA1-980-HRP detects c-myc protein and c-myc tagged proteins.
- Conjugate
- Horseradish Peroxidase
- Antibody clone number
- 9E10
- Concentration
- 1 mg/mL
Submitted references A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation.
Proteolytic degradation of regulator of G protein signaling 2 facilitates temporal regulation of G(q/11) signaling and vascular contraction.
HCMV-encoded glycoprotein M (UL100) interacts with Rab11 effector protein FIP4.
Liu Z, Nailwal H, Rector J, Rahman MM, Sam R, McFadden G, Chan FK
Immunity 2021 Feb 9;54(2):247-258.e7
Immunity 2021 Feb 9;54(2):247-258.e7
Proteolytic degradation of regulator of G protein signaling 2 facilitates temporal regulation of G(q/11) signaling and vascular contraction.
Kanai SM, Edwards AJ, Rurik JG, Osei-Owusu P, Blumer KJ
The Journal of biological chemistry 2017 Nov 24;292(47):19266-19278
The Journal of biological chemistry 2017 Nov 24;292(47):19266-19278
HCMV-encoded glycoprotein M (UL100) interacts with Rab11 effector protein FIP4.
Krzyzaniak MA, Mach M, Britt WJ
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1439-57
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1439-57
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of c-Myc was performed by loading 50 µg of NCCIT and Jurkat lysates, and 10 µg of Myc-MYCBP-containing cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated c-Myc monoclonal antibody (Product # MA1-980-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) at an exposure time of 2 min (NCCIT and Jurkat) and 3 sec (Myc-MYCBP). Results show a band at ~57 kDa (NCCIT and Jurkat) and 16 kDa (Myc-MYCBP) as well as a 2 unknown bands at 35-40 kDa.
- Conjugate
- Horseradish Peroxidase
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Myc Tag Monoclonal Antibody (Product # MA1-980-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293 lysates: untransfected, 40 µg (Lane 1), empty vector control, 60 µg (Lane 2), Myc-p65-V5, 60 µg (Lane 3), Myc-p65-V5, 40 µg (Lane 4), Myc-p65-V5, 20 µg (Lane 5), Myc-p65-V5, 10 µg (Lane 6), p65-HA, 60 µg (Lane 7) and 25 ng of Positope (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). A ~65 kDa band corresponding to Myc-p65-V5 was observed in HEK293E transfected lysates on probing with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Positope (Product # R90050) is a 53 kDa recombinant protein consisting of multiple epitope tags but lacks HA tag, which has been used as a positive control for c-Myc tag detection. No cross-reactivity was seen with HA-tagged p65 expressing lysate.
- Conjugate
- Horseradish Peroxidase
