F48482

antibody from NSJ Bioreagents

Targeting: MYC bHLHe39, c-Myc, MYCC

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

F48482

Provider

NSJ Bioreagents

Product name

Phospho c-Myc Antibody (pT58)

Antibody type

Polyclonal

Antigen

This phospho c-Myc antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT58 of human MYC.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the phospho c-Myc antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of phospho c-Myc antibody and human TPA activated HeLa cells/lysate (0: without TPA; 1: 60ug/ml TPA-15min; 2: 60ug/ml-30min; 3: 60ug/ml-45min; 4: 125ug/ml-15min; 5: 125ug/ml-30min; 6: 125ug/ml-45min; 7: 250ug/ml-15min; 8: 250ug/ml-30min; 9: 250ug/ml-45min)

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Dot blot analysis of phospho c-Myc antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.