MA1-7629

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-7629

Provider

Invitrogen Antibodies

Product name

p53 Monoclonal Antibody (BD53-12)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-7629 detects p53 from human, rat and mouse samples.

Antibody clone number

BD53-12

Concentration

1 mg/mL

References

Submitted references

The effect of vitamin C administration on monosodium glutamate induced liver injury. An experimental study.

El-Meghawry El-Kenawy A, Osman HE, Daghestani MH
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2013 Jul;65(5):513-21

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The effects of PDE5 inhibitory drugs on renal ischemia/reperfusion injury in rats.

Küçük A, Yucel M, Erkasap N, Tosun M, Koken T, Ozkurt M, Erkasap S
Molecular biology reports 2012 Oct;39(10):9775-82

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Hepatoprotective effect of coenzyme Q10 in rats with acetaminophen toxicity.

Fouad AA, Jresat I
Environmental toxicology and pharmacology 2012 Mar;33(2):158-67

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X-irradiation reduces the proliferation of astrocytes by cell cycle arrest.

Wang Q, Xu Y, Xie MJ, Yu ZY, Qin YY, Wang W, Zhu Z
Neuroscience letters 2011 Jul 1;498(1):78-83

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Chk1 deficiency in the mouse small intestine results in p53-independent crypt death and subsequent intestinal compensation.

Greenow KR, Clarke AR, Jones RH
Oncogene 2009 Mar 19;28(11):1443-53

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Protective effects of doxycycline in ischemia/reperfusion injury on kidney.

Kucuk A, Kabadere S, Tosun M, Koken T, Kinaci MK, Isikli B, Erkasap N
Journal of physiology and biochemistry 2009 Jun;65(2):183-91

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Immunohistochemical study of proteins linked to apoptosis in rat fetal kidney cells following prepregnancy adriamycin administration in the mother.

Pedrycz A, Czerny K
Acta histochemica 2008;110(6):519-23

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p53 Expression and apoptosis in liver and spleen during CO2 pneumoperitoneum.

Arikan Y, Tosun M, Saykol V, Kalkan S, Erdem S
Langenbeck's archives of surgery 2008 Nov;393(6):877-82

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of p53 was performed using 70% confluent log phaseT-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with p53 Monoclonal Antibody (4A8) (Product # MA1-7629) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel f represents SK-OV-3 cells as negative control, showing no p53 staining. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous p53 protein at specific gene loci using Anti-p53 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-p53 Mouse Monoclonal Antibody (Product # MA1-7629, 4 µg) on sheared chromatin from 2 million Etoposide-treated A-431 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the CDKN1A and CMBL genes (active) and SAT2 satellite repeats (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.