GTX117515

antibody from GeneTex

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX117515

Provider

GeneTex

Proper citation

GeneTex Cat#GTX117515, RRID:AB_10617499

Product name

p53 (mono-methyl Lys372) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

Hibernation impacts lysine methylation dynamics in the 13-lined ground squirrel, Ictidomys tridecemlineatus.

Watts AJ, Storey KB
Journal of experimental zoology. Part A, Ecological and integrative physiology 2019 Apr;331(4):234-244

---

Histone methylation in the freeze-tolerant wood frog (Rana sylvatica).

Hawkins LJ, Storey KB
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 2018 Jan;188(1):113-125

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE GTX117515 diluted at 1:500

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53 (mono-methyl Lys372)(GTX117515) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

p53 (mono-methyl Lys372) antibody detects p53 (mono-methyl Lys372) protein at cytoplasm and nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. p53 (mono-methyl Lys372) antibody (GTX117515) diluted at 1:500.