MA1-19424

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19424

Provider

Invitrogen Antibodies

Product name

Phospho-p53 (Ser392) Monoclonal Antibody (FP3.2 [FPS392])

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

For IHC staining, high temperature antigen retrieval (microwave, pressure cooker) is recommended, using 10 mM citrate buffer, pH 6.0, or 1 mM EDTA-NaOH buffer, pH 8.0.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

FP3.2 [FPS392]

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of phosphorylated human p53 using mouse monoclonal antibody FP3.2 (purified) (Product # MA1-19424) in T47D cell line, which expresses p53. A) Phosphorylated p53 detected in T47D cell line. B) Absence of phosphorylated signal of p53 in dephosphorylated T47 cells (pretreated with calf intestinal alkaline phosphatase).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-p53 (Ser392) using a monoclonal antibody (Product # MA1-19424).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-p53 (Ser392) using a monoclonal antibody (Product # MA1-19424).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). A – anti-p53 (total); B – anti-p53 (phospho Ser392) Monoclonal antibody (Product # MA1-19424). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.