MA1-183

antibody from Invitrogen Antibodies

Targeting: ARRB1 ARR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-183

Provider

Invitrogen Antibodies

Product name

beta Arrestin 1 Monoclonal Antibody (4E11B1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA1-183 detects beta Arrestin 1 protein from human samples at approximately 55 kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4E11B1

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ARRB1 was performed by loading 10-20 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. ARRB1 was detected at ~55 kDa using an ARRB1 mouse monoclonal antibody (Product # MA1-183) at a concentration of 1 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least one hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FXR2 in MCF7 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with an ARRB1 mouse monoclonal antibody (Product # MA1-183) at a concentration of 2.5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® Plus 488 conjugate (Product # A32723) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.