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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- GTX128170 - Provider product page
- Provider
- GeneTex
- Product name
- CDC25C antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC25C antibody detects CDC25C protein by Western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extractC. 30 ?g K562 whole cell lysate/extractD. 30 ?g NCI-H929 whole cell lysate/extract10 % SDS-PAGECDC25C antibody (GTX128170) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC25C antibody detects CDC25C protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract (untreated)B. 30 ?g HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)10 % SDS-PAGECDC25C antibody (GTX128170) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody (GTX128170) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody (GTX128170) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC25C antibody detects CDC25C protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDC25C protein stained by CDC25C antibody (GTX128170) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
