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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- TA319229 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Cyclin B1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Cyclin B1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CCNB1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 80 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB analysis using anti-cyclin B antibody shows detection of human Cyclin B present in asynchronous HN30 cell lysates.HN30 cells are from head and neck cancer tumors that over express cyclin B and D. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead).? The blot was incubated with a 1:500 dilution of the antibody.Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
- Validation comment
- IHC
