NBP2-44688-0.1mg

antibody from Novus Biologicals

Targeting: CCNB1 CCNB

Immunocytochemistry

Flow cytometry

Antibody data

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Validation data

Product number: NBP2-44688-0.1mg - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal Cyclin B1 Antibody
Antibody type: Monoclonal
Description: Protein A or G purified. It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Concentration: 0.2 mg/ml
Storage: Store at 4C.

CCNB1 protein structure - NBP2-44688-0.1mg shown in red.