MA5-14319

antibody from Invitrogen Antibodies

Targeting: CCNB1 CCNB

Provider product page for MA5-14319

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [24]
Comments [0]
Validations
Western blot [2]
Other assay [4]

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Product number: MA5-14319 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cyclin B1 Monoclonal Antibody (GNS1)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-14319 targets Cyclin B1 in FACS, IF, IP, and WB applications and shows reactivity with Hamster, Human, and mouse samples.
Antibody clone number: GNS1
Concentration: 0.2 mg/mL

CCNB1 protein structure - MA5-14319 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Cyclin B1 using Cyclin B1 Monoclonal Antibody (Product # MA5-14319) on Native Human LS174T Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Cellular and molecular consequences of sarcomere assembly (A) Representative immunofluorescence images of wild-type (WT) control and cardiac troponin T knockout (cTnT-KO) CMs stained for alpha actinin (green; sarcomere Z-disk) and DAPI (blue; nuclei). KO of troponin, either cTnT-KO or double KO of skeletal and cardiac troponin I (TnI-DKO), leads to lack of striated sarcomere Z-disks. Scale bar, 10 mum. (B and C) Flow cytometry of Hoechst-stained CMs demonstrates decreased polyploidy in (B) cTnT-KO and (C) TnI-DKO CMs relative to WT. (D) Flow cytometry of CCNB1-stained WT and TnI-DKO CMs demonstrates increased proportion of CCNB1 + CMs with TnI-DKO, which reduces with age. (E and F) Representative immunoblots (E) with (F) quantification of protein lysates from WT and cTnT-KO CMs probed for cTnT, p53, p21, phospho-CCNB1, and GAPDH. (G and H) Representative immunoblots (G) with (H) quantification of protein lysates from WT and TnI-DKO CMs probed for TnI (cardiac and skeletal), p53, p21, p-CCNB1, and GAPDH. (I and J) Representative immunoblots (I) with (J) quantification of protein lysates from day 20 WT CMs treated with verapamil from day 9 and probed for p53, p21, p-CCNB1, and GAPDH. (K) Anti-p53 ChIP-qPCR of WT and cTnT-KO CMs targeting previously reported p53-bound ChIP-seq peaks directly upstream of CDKN1A (), which demonstrates decreased p53 binding of CDKN1A in cTnT-KO CMs at three separate genomic sites (see Figure 3D ). (L and M) Representative immunoblots prob