46-9326-41
antibody from Invitrogen Antibodies
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-9326-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD326 (EpCAM) Monoclonal Antibody (1B7), PerCP-eFluor 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM. Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1B7 antibody has been pre-titrated and tested by flow cytometric analysis of A549 human lung carcinoma cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level.
TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
The isolation and characterization of CTC subsets related to breast cancer dormancy.
NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.
GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.
Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.
Slow-cycling therapy-resistant cancer cells.
Exploration of target molecules for prostate cancer gene therapy.
Pode-Shakked N, Gershon R, Tam G, Omer D, Gnatek Y, Kanter I, Oriel S, Katz G, Harari-Steinberg O, Kalisky T, Dekel B
Stem cell reports 2017 Jul 11;9(1):279-291
Stem cell reports 2017 Jul 11;9(1):279-291
TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
Huang SC, Wei PC, Hwang-Verslues WW, Kuo WH, Jeng YM, Hu CM, Shew JY, Huang CS, Chang KJ, Lee EY, Lee WH
EMBO molecular medicine 2017 Dec;9(12):1660-1680
EMBO molecular medicine 2017 Dec;9(12):1660-1680
The isolation and characterization of CTC subsets related to breast cancer dormancy.
Vishnoi M, Peddibhotla S, Yin W, T Scamardo A, George GC, Hong DS, Marchetti D
Scientific reports 2015 Dec 3;5:17533
Scientific reports 2015 Dec 3;5:17533
NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.
Kottakis F, Foltopoulou P, Sanidas I, Keller P, Wronski A, Dake BT, Ezell SA, Shen Z, Naber SP, Hinds PW, McNiel E, Kuperwasser C, Tsichlis PN
Cancer research 2014 Jul 15;74(14):3935-46
Cancer research 2014 Jul 15;74(14):3935-46
GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.
Chou J, Lin JH, Brenot A, Kim JW, Provot S, Werb Z
Nature cell biology 2013 Feb;15(2):201-13
Nature cell biology 2013 Feb;15(2):201-13
Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.
Paik DY, Janzen DM, Schafenacker AM, Velasco VS, Shung MS, Cheng D, Huang J, Witte ON, Memarzadeh S
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2487-97
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2487-97
Slow-cycling therapy-resistant cancer cells.
Moore N, Houghton J, Lyle S
Stem cells and development 2012 Jul 1;21(10):1822-30
Stem cells and development 2012 Jul 1;21(10):1822-30
Exploration of target molecules for prostate cancer gene therapy.
Suzuki K, Nakamura K, Kato K, Hamada H, Tsukamoto T
The Prostate 2007 Aug 1;67(11):1163-73
The Prostate 2007 Aug 1;67(11):1163-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the A549 human lung carcinoma cell line with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD326 (EpCAM) PerCP-eFluor® 710 (purple histogram). Total viable cells were used for analysis.