MA5-15162

antibody from Invitrogen Antibodies

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15162

Provider

Invitrogen Antibodies

Product name

MEK1/MEK2 Monoclonal Antibody (E.901.7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Isotype

IgG

Antibody clone number

E.901.7

Vial size

100 µL

Storage

-20°C

References

Submitted references

CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons.

Brustovetsky T, Khanna R, Brustovetsky N
Cells 2021 Oct 17;10(10)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), H-1975 (Lane 2), HCT 116 (Lane 3), HT-29 (Lane 4), A-431 (Lane 5), A549 (Lane 6) MDA-MB-231 (Lane 7), U-87 MG (Lane 8), MCF7 (Lane 9), Raji (Lane 10) and NIH/3T3 (Lane 11). The blot was probed with Anti-MEK1/MEK2 Rabbit Monoclonal Antibody (Product # MA5-15162, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 45 kDa band corresponding to MEK1/MEK2 was detected across all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard  (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Interaction of CRMP2 with brain mitochondria. ( A - C ) Immunocytochemistry staining with anti-CRMP2 antibody ( A , red) and visualization of mitochondria with mitochondrially targeted enhanced yellow fluorescent protein (mito-eYFP) ( B , green) illustrates co-localization of CRMP2 and mitochondria (yellow, C ) in cultured striatal neuron from FVB/NJ mouse. ( D , E ), CRMP2 binds to synaptic brain mitochondria isolated from FVB/NJ mice. Mitochondria were purified either on discontinuous 26/40% Percoll gradient ( D ) or on continuous 30% Percoll gradient ( E ) as described in the Materials and Methods. H: homogenate; C: cytosolic fraction; Mtc: mitochondrial fraction. MEK1/2, calnexin, beta-tubulin, Histone H3, and COX IV are cytosolic, ER, microtubule, nuclear, and mitochondrial markers, respectively. Representative western blots from 3 independent experiments are shown.