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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB9600 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9600, RRID:AB_1710028
- Product name
- MAP2K1/MAP2K2 (phospho S218/S222) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide corresponding to residues surrounding S218/S222 of human MAP2K1/MAP2K2 .
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 uL
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mechanisms of regulating the Raf kinase family.
Molecular psychology: roles for the ERK MAP kinase cascade in memory.
The MAP kinase cascade. Discovery of a new signal transduction pathway.
Chong H, Vikis HG, Guan KL
Cellular signalling 2003 May;15(5):463-9
Cellular signalling 2003 May;15(5):463-9
Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Adams JP, Sweatt JD
Annual review of pharmacology and toxicology 2002;42:135-63
Annual review of pharmacology and toxicology 2002;42:135-63
The MAP kinase cascade. Discovery of a new signal transduction pathway.
Ahn NG
Molecular and cellular biochemistry 1993 Nov;127-128:201-9
Molecular and cellular biochemistry 1993 Nov;127-128:201-9
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot of NIH/3T3 cell lysates showing specific immunolabeling of the ~45k MAP2K1/MAP2K2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95k was also observed.
