Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36823 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MEK1/MEK2 (Ser218, Ser222) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 43 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-MEK1/MEK2 (Ser218, Ser222) in Lane 1: A549 cell lysate treated with PMA (100 nM,30mins), Lane 2: Raw264.7 cell lysate treated with PMA (100 nM,30mins), Lane 3: NIH-3T3 cell lysate treated with PMA (100 nM,30mins), Lane 4: PC12 cell lysate treated with PMA (100 nM,30mins). Samples were incubated with Phospho-MEK1/MEK2 (Ser218, Ser222) polyclonal antibody (Product # PA5-36823) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-MEK1 pSer218+MEK2 pSer222 using Phospho-MEK1 pSer218+MEK2 pSer222 polyclonal antibody (Product # PA5-36823) at a dilution of 1:500. Lane 1: A549 cell lysate treated with PMA (100nM, 30min), Lane 2: Raw264.7 cell lysate treated with PMA (100nM, 30min), Lane 3: NIH-3T3 cell lysate treated with PMA (100nM, 30min), Lane 4: PC12 cell lysate treated with PMA (100nM, 30min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-MEK1 pSer218+MEK2 pSer222 in paraffin-embedded human breast carcinoma using Phospho-MEK1 pSer218+MEK2 pSer222 polyclonal antibody (Product # PA5-36823).