Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA321906 - Provider product page
- Provider
- OriGene
- Product name
- Anti-MAP2K2 (Phospho-Thr394) Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Anti-MAP2K2 (Phospho-Thr394) Rabbit Polyclonal Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MAP2K2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted band size: 44 kDa . Positive control: HepG2 and Hela cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HepG2 cells untreated with UV lysate Lane2: HepG2 cells treated with UV lysate Lane3: Hela cells untreated with UV lysate Lane4: Hela cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using MAP2K2 (Phospho-Thr394) antibody at dilution 1/100. (Original magnification:?200)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using MAP2K2 (Phospho-Thr394) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
- Validation comment
- IHC
