GTX132329

antibody from GeneTex

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX132329

Provider

GeneTex

Product name

PARP (cleaved Asp214) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP (cleaved Asp214) antibody (GTX132329) diluted at 1:3000.

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

42347

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HCT116 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 (cleaved Asp214) antibody (GTX132329) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP (cleaved Asp214) antibody (GTX132329) diluted at 1:3000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PARP1 (cleaved Asp214) antibody detects PARP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP1 protein stained by PARP1 (cleaved Asp214) antibody (GTX132329) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.