12-6668-42

antibody from Invitrogen Antibodies

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12-6668-42

Provider

Invitrogen Antibodies

Product name

PARP1 (cleaved Asp214) Monoclonal Antibody (HLNC4), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa.

Conjugate

Yellow dye

Antibody clone number

HLNC4

Concentration

5 µL/Test

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were left unstimulated (left) or stimulated for 20 hours with Anti-Human CD95 (APO-1/Fas) Functional Grade Purified (Product # 16-0958-81) coated at 5 µg/mL in a 24-well culture plate (right). The stimulated cells were then harvested and stained sequentially with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) and the Annexin V Apoptosis Detection Kit eFluor® 450 (Product # 88-8006-72), then fixed and permeabilized with the Foxp3/transcription Factor Staining Buffer Set (Product # 00-5523-00) and stained with Anti-Human PARP1 (Cleaved) PE. Total viable cells (Fixable Viability Dye eFluor® 780 negative) were used for analysis.

Conjugate

Yellow dye