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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- MA5-18072 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Methyl-PP2A alpha (Leu309) Monoclonal Antibody (2A10)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody recognizes the C-terminal methylated form of PP2A (C subunit). The c-terminal sequence, as well as the carboxy-methylation, is 100% conserved in human, mouse and yeast.
- Antibody clone number
- 2A10
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Methyl-PP2A alpha (Leu309) in two yeast strains using a Methyl-PP2A alpha (Leu309) monoclonal antibody (Product # MA5-18072). Native cultures from yeast deletion strains expressing the HA-tagged PP2AC (PPH21) were subjected to IP against the HA tag: strain PPE1-delta (left) which lacks the PP2A methylesterase (PPE1) and accumulates methylated PPH21, and PPM1-delta which lacks the PP2A methyltransferase and contains no methylated PPH21. The lysates were boiled in Laemmli buffer, separated by SDS-PAGE and transfered to a nitrocellulose membrane. Lane 5 shows a control incubated with an anti-HA tag monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Methyl-PP2A alpha (Leu309) in two yeast strains using a Methyl-PP2A alpha (Leu309) monoclonal antibody (Product # MA5-18072). Native cultures from yeast deletion strains expressing the HA-tagged PP2AC (PPH21) were subjected to IP against the HA tag: strain PPE1-delta (left) which lacks the PP2A methylesterase (PPE1) and accumulates methylated PPH21, and PPM1-delta which lacks the PP2A methyltransferase and contains no methylated PPH21. The lysates were boiled in Laemmli buffer, separated by SDS-PAGE and transfered to a nitrocellulose membrane. Lane 5 shows a control incubated with an anti-HA tag monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Methyl-PP2A alpha (Leu309) in two yeast strains using a Methyl-PP2A alpha (Leu309) monoclonal antibody (Product # MA5-18072). Native cultures from yeast deletion strains expressing the HA-tagged PP2AC (PPH21) were subjected to IP against the HA tag: strain PPE1-delta (left) which lacks the PP2A methylesterase (PPE1) and accumulates methylated PPH21, and PPM1-delta which lacks the PP2A methyltransferase and contains no methylated PPH21. The lysates were boiled in Laemmli buffer, separated by SDS-PAGE and transfered to a nitrocellulose membrane. Lane 5 shows a control incubated with an anti-HA tag monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Methyl-PP2A alpha (Leu309) in two yeast strains using a Methyl-PP2A alpha (Leu309) monoclonal antibody (Product # MA5-18072). Native cultures from yeast deletion strains expressing the HA-tagged PP2AC (PPH21) were subjected to IP against the HA tag: strain PPE1-delta (left) which lacks the PP2A methylesterase (PPE1) and accumulates methylated PPH21, and PPM1-delta which lacks the PP2A methyltransferase and contains no methylated PPH21. The lysates were boiled in Laemmli buffer, separated by SDS-PAGE and transfered to a nitrocellulose membrane. Lane 5 shows a control incubated with an anti-HA tag monoclonal antibody.
