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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00002185-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002185-M01, RRID:AB_519004
- Product name
- PTK2B monoclonal antibody (M01), clone 1F9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PTK2B.
- Antigen sequence
VYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPK
PSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLT
SPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQ
PSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQW
LRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPP
QKPPRLGAQSIQPTA- Isotype
- IgG
- Antibody clone number
- 1F9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.Lane 1: PTK2B transfected lysate(115.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PTK2B is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PTK2B transfected lysate using anti-PTK2B monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PTK2B MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
