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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- TA319556 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal anti-STAT2 antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal anti-STAT2 antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- STAT2
- Antibody clone number
- 19G8.H2.H6
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HeLa whole cell lysate was loaded at 1 ug. The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with anti-Stat2 antibody in 3% BSA/TBST at 1:1,000 overnight at 4C. After washing, blot was incubated with HRP Rb a-Ms IgG (p/n 610-4302) antibody in blocking buffer (p/n MB-070) for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc? 4000 MP.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- anti-Stat2 monoclonal antibody was used at 2.5 ug/mL to detect Stat2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (image). Expression of Stat2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
- Validation comment
- IHC
