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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- R30938 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- STAT5a Antibody
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of human STAT5a (YNMYPQNPDHVLDQDGE) was used as the immunogen for this STAT5a antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the STAT5a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of STAT5a antibody and Lane 1: HeLa; 2: COLO320; 3: Jurkat; 4: CEM cell lysate. Predicted size ~91KD
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Jurkat cells with STAT5a antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT5a antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-2 OS cells with STAT5a antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT5a antibody.
