PA5-29709

antibody from Invitrogen Antibodies

Targeting: STAT5A MGF, STAT5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29709

Provider

Invitrogen Antibodies

Product name

STAT5 alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, OECM-1, SCC-9.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of STAT5 alpha was performed by separating 12.7 µg of whole cell extract by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a STAT5 alpha Polyclonal Antibody (Product # PA5-29709) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of STAT5 alpha was performed by separating 22.5 µg of whole cell extract by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a STAT5 alpha Polyclonal Antibody (Product # PA5-29709) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using STAT5 alpha Polyclonal Antibody (Product # PA5-29709). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2 . 7.5% SDS PAGE. STAT5 alpha Polyclonal Antibody (Product # PA5-29709) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of STAT5A in paraformaldehyde-fixed HeLa cells using a STAT5A polyclonal antibody (Product # PA5-29709) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

STAT5 alpha Polyclonal Antibody detects STAT5A protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. STAT5 alpha Polyclonal Antibody (Product # PA5-29709) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

STAT5 alpha Polyclonal Antibody detects STAT5A protein at cytosol and nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. STAT5 alpha Polyclonal Antibody (Product # PA5-29709) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.