Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-34737 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFkB p65 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, GFP-human NF-kappa-B-transfected 293T.
- Concentration
- 1.46 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p65 using Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-34737) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p65 using A) 30 µg GL261 whole cell lysate (B) 30 µg C8D30 whole cell lysate and C) 30 µg NIH-3T3 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-34737) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p65 using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-34737) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 15 µg of non-transfected (-) and NFkB p65 -transfected (+) (including GFP-tag) 293T whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NFkB p65 in paraformaldehyde-fixed U2OS cells using a NFkB p65 polyclonal antibody (Product # PA5-34737) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NFkB p65 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 Polyclonal Antibody (Product # PA5 34737) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded mouse cervix tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded rat liver tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded mouse kidney tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded mouse lung tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFkB p65 was performed in paraffin-embedded rat lung tissue using NFkB p65 Polyclonal Antibody (Product # PA5-34737) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFkB p65 Polyclonal Antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 µg of NFkB p65 Polyclonal Antibody (Product # PA5-34737) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 Polyclonal Antibody (Product # PA5-34737) diluted at 1:1,000.
