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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-37556 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-4EBP1 (Thr37) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous level of 4E-BP1 only when phosphorylated at threonine 37.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/mL, 30 min), using 4E-BP1 (pThr36) polyclonal antibody (Product # PA5-37556) (Lanes 3-4) and an alternate 4E-BP1 polyclonal antibody (Lanes 1-2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-4EBP1 (Thr37) in paraffin-embedded Human breast carcinoma tissue using Phospho-4EBP1 (Thr37) Polyclonal Antibody (Product # PA5-37556).
