MA1-24598

antibody from Invitrogen Antibodies

Targeting: CRP PTX1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Recommended by provider)

Antibody Data

Product number

MA1-24598

Provider

Invitrogen Antibodies

Product name

CRP Monoclonal Antibody (C1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA1-24598 detects C Reactive Protein from human samples.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

C1

Vial size

100 µg

Concentration

7.6 mg/mL

Storage

4° C

References

Submitted references

Immunolocalization of cellular retinoic acid binding protein to Müller cells and/or a subpopulation of GABA-positive amacrine cells in retinas of different species.

Milam AH, De Leeuw AM, Gaur VP, Saari JC
The Journal of comparative neurology 1990 Jun 1;296(1):123-9

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CRP was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CRP Monoclonal Antibody (C1) (Product # MA1-24598, 5 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000 dilution), for 45 minutes at room temperature (Panels a,e,i: Green). Nuclei (Panels b,f,j: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panels c,g,k: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panels d,h,l represents the merged images showing cytoplasmic localization upon treatment with IL-6 and PTI. Panel (a-d) shows representative HepG2 control cells, whereas Panel (e-h) represent HepG2 with Protein Transport Inhibitor Cocktail (PTI)(500X) (1X, 4 hours) treatment. Similarly, panel (i-l) represent HepG2 cells treated with IL-6 and Protein Transport Inhibitor Cocktail (PTI)(500X) (10 ng/mL, 1X; 24 hours, 4 hours). The images were captured at 60X magnification.