Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF3197 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal AMPK alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat AMPK alpha 1 in Western blots. The antibody does not cross-react with recombinant human AMPK alpha 2.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Disrupted structure and aberrant function of CHIP mediates the loss of motor and cognitive function in preclinical models of SCAR16.
Modulating the therapeutic response of tumours to dietary serine and glycine starvation.
AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo.
Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons.
Shi CH, Rubel C, Soss SE, Sanchez-Hodge R, Zhang S, Madrigal SC, Ravi S, McDonough H, Page RC, Chazin WJ, Patterson C, Mao CY, Willis MS, Luo HY, Li YS, Stevens DA, Tang MB, Du P, Wang YH, Hu ZW, Xu YM, Schisler JC
PLoS genetics 2018 Sep;14(9):e1007664
PLoS genetics 2018 Sep;14(9):e1007664
Modulating the therapeutic response of tumours to dietary serine and glycine starvation.
Maddocks ODK, Athineos D, Cheung EC, Lee P, Zhang T, van den Broek NJF, Mackay GM, Labuschagne CF, Gay D, Kruiswijk F, Blagih J, Vincent DF, Campbell KJ, Ceteci F, Sansom OJ, Blyth K, Vousden KH
Nature 2017 Apr 19;544(7650):372-376
Nature 2017 Apr 19;544(7650):372-376
AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo.
Domise M, Didier S, Marinangeli C, Zhao H, Chandakkar P, Buée L, Viollet B, Davies P, Marambaud P, Vingtdeux V
Scientific reports 2016 May 27;6:26758
Scientific reports 2016 May 27;6:26758
Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons.
Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y
The Journal of biological chemistry 2012 Jun 8;287(24):19985-96
The Journal of biological chemistry 2012 Jun 8;287(24):19985-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat AMPK alpha 1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPK alpha 1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human. Mouse, and Rat AMPKa1 by Western Blot. Detection of Human. Mouse, and Rat AMPKa1 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- AMPK alpha 1 in Human Liver. AMPK alpha 1 was detected in immersion fixed paraffin-embedded sections of human liver using 5 µg/mL Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows secondary antibody only control experiment. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.