GTX630200

antibody from GeneTex

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Enhanced validation)

Antibody Data

Product number

GTX630200

Provider

GeneTex

Product name

mTOR antibody [GT778]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT778] (GTX630200) diluted at 1:500.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41512

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mTOR antibody [GT778] detects mTOR protein by western blot analysis. A Jurkat whole cell lysate/extractBRaji whole cell lysate/extract C NCI-H929 whole cell lysate/extract5 % SDS-PAGEmTOR antibody [GT778] (GTX630200) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mTOR antibody [GT778] detects mTOR protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extractC. 30 £gg HeLa whole cell lysate/extractD. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGEmTOR antibody [GT778] (GTX630200) dilution: 1:500

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT778] (GTX630200) diluted at 1:500.