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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM50059PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti NCK1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human NCK1 (1-377aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT6B3
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCK1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
