H00004690-M01

antibody from Abnova Corporation

Targeting: NCK1 NCK, NCKalpha

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004690-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004690-M01, RRID:AB_463964

Product name

NCK1 monoclonal antibody (M01), clone 1A1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant NCK1.

Antigen sequence

NLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDV
IEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNP
LTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTR
HQAEM


Isotype

IgG

Antibody clone number

1A1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NCK1 monoclonal antibody (M01), clone 1A1 Western Blot analysis of NCK1 expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody (M01), clone 1A1.Lane 1: NCK1 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NCK1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol