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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX60832 - Provider product page
- Provider
- GeneTex
- Product name
- HDAC3 antibody [GT9080]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- ICC/IF analysis of HeLa cells using HDAC3 antibody (red) and DAPI. Cells were fixed with 4% formaldehyde for 10¡¦ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ChIP was performed with HeLa extracts and either 5 £gg of HDAC3 antibody or control IgG. The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter, c-fos promoter and the coding region of p21, a known target gene of HDAC3.
