PA5-20008

antibody from Invitrogen Antibodies

Targeting: BBC3 JFY1, PUMA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20008

Provider

Invitrogen Antibodies

Product name

PUMA alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The PA5-20008 immunogen is located within the first 50 amino acids of PUMA. The immunogenic sequence is identical between the A and B forms of PUMA.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation of PUMA in K562 Cells. Loading: 15 µg of lysates per lane. Antibodies: PUMA Polyclonal Antibody (Product # PA5-20008) (2 µg/mL), 1 h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of K562 cells using a PUMA polyclonal antibody (Product # PA5-20008) at a 10 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with PUMA Polyclonal Antibody (Product # PA5-20008) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red) and DAPI staining (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with PUMA Polyclonal Antibody (Product # PA5-20008) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red) and DAPI staining (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with PUMA Polyclonal Antibody (Product # PA5-20008) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red). Image showing cytosol staining on K562 cells

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PUMA Polyclonal Antibody (Product # PA5-20008) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast tissue using PUMA Polyclonal Antibody (Product # PA5-20008) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.