Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-36618 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ASK1 (Ser83) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 155 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-ASK1 (Ser83) in Lane 1: A549 whole cell lysate (40 µg), Lane 2: Panc1 whole cell lysate (40 µg), Lane 3: H1792 whole cell lysate (30 µg). Samples were incubated with Phospho-ASK1 (Ser83) polyclonal antibody (Product # PA5-36618) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Phospho-ASK1 (Ser83) Polyclonal Antibody (Product # PA5-36618) and a 170 kDa band corresponding to Mitogen-activated protein kinase kinase kinase 5 was observed across tested panel. Whole cell extracts (40 µg lysate) of MCF7 (Lane 1), MCF7 treated with Nocodazole 200 ng/mL for 16 hours (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-ASK1 (Ser83) in Lane 1: A549 whole cell lysate (40 µg), Lane 2: Panc1 whole cell lysate (40 µg), Lane 3: H1792 whole cell lysate (30 µg). Samples were incubated with Phospho-ASK1 (Ser83) polyclonal antibody (Product # PA5-36618) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-ASK1/MAP3K5 pSer83 using Phospho-ASK1/MAP3K5 pSer83 polyclonal antibody (Product # PA5-36618) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha, Lane 2: PC12 cell lysate treated with TNF-alpha, Lane 3: sp2/0 cell lysate treated with TNF-alpha.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-ASK1 (Ser83) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-ASK1 (Ser83) polyclonal antibody (Product # PA5-36618) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-ASK1/MAP3K5 pSer83 in paraffin-embedded human breast carcinoma using Phospho-ASK1/MAP3K5 pSer83 polyclonal antibody (Product # PA5-36618) at a dilution of 1:100.