MA1-21460

antibody from Invitrogen Antibodies

Targeting: XPA XP1, XPAC

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-21460

Provider

Invitrogen Antibodies

Product name

XPA Monoclonal Antibody (5A2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive controls of LS174T, MCF-7 cells or human tonsil suggested. Works on pure and partially purified protein but not on crude cell extract.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5A2

Vial size

100 µg

Concentration

1.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

XPA Enhances Temozolomide Resistance of Glioblastoma Cells by Promoting Nucleotide Excision Repair.

Dai W, Wu A, Li Y, Yu G, Yan X
Cell transplantation 2022 Jan-Dec;31:9636897221092778

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Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases.

Marotti B, Rocco A, De Colibus P, Compare D, de Nucci G, Staibano S, Tatangelo F, Romano M, Nardone G
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2008 Apr;40(4):240-7

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Modulation of the infant immune responses by the first pertussis vaccine administrations.

Mascart F, Hainaut M, Peltier A, Verscheure V, Levy J, Locht C
Vaccine 2007 Jan 4;25(2):391-8

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Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.

Mascart F, Verscheure V, Malfroot A, Hainaut M, Piérard D, Temerman S, Peltier A, Debrie AS, Levy J, Del Giudice G, Locht C
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1504-9

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Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.

Mascart F, Verscheure V, Malfroot A, Hainaut M, Piérard D, Temerman S, Peltier A, Debrie AS, Levy J, Del Giudice G, Locht C
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1504-9

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Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus.

Hancock GE, Scheuer CA, Sierzega R, Pryharski KS, McBride JT, Watelet LF, Tebbey PW, Smith JD
The Journal of infectious diseases 2001 Dec 15;184(12):1589-93

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Temozolomide-resistant GBM cells exhibits a high expression level of XPA. (A) Representative western blot analysis of NER-related proteins and GAPDH in U87 cells, U87-R cells, U251 cells and U251-R cells. (B) qRT-PCR analysis of NER-related genes and GAPDH in U87 cells, U87-R cells, U251 cells and U251-R cells. *** P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Silencing XPA attenuates temozolomide resistance. (A) The distribution of IC50 scores in different groups, where the horizontal axis represents samples of different groups, and the vertical axis represents the distribution of IC50 scores, *** P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. XPA silencing increases temozolomide sensitivity in vivo . (A) U87 and U87-R cells stably transfected with shCon or shXPA, and subsequently injected into nude mice. Tumors were excised and volume was calculated every week. Representative images of isolated tumors were displayed. (B) The tumor inhibition rates according to the weight of explanted tumors in each experimental group. *** P