AF3416

antibody from Novus Biologicals

Targeting: XPA XP1, XPAC

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3416

Provider

Novus Biologicals

Product name

Goat Polyclonal XPA Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human XPA in Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Sirtuin 1-mediated deacetylation of XPA DNA repair protein enhances its interaction with ATR protein and promotes cAMP-induced DNA repair of UV damage.

Jarrett SG, Carter KM, Bautista RM, He D, Wang C, D'Orazio JA
The Journal of biological chemistry 2018 Dec 7;293(49):19025-19037

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human XPA by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3416) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPA at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.